한빛사
>
한빛사논문
한빛사논문
- 조회215
Adv. Sci., Jan 31 2025, | https://doi.org/10.1002/advs.202412621
Osteoblast-Derived Mitochondria Formulated with Cationic Liposome Guide Mesenchymal Stem Cells into Osteogenic Differentiation
Hye-Ryoung Kim 1 †, Seonjeong Woo 2 †, Hui Bang Cho 1, Sujeong Lee 1, Chae Won Cho 1, Ji-In Park 1, Seulki Youn 1, Gyuwon So 1, Sumin Kang 1, Sohyun Hwang 2 3 *, Hye Jin Kim 1 *, Keun-Hong Park 1 *
1School of Bioconvergence, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si, 13488, Republic of Korea.
2Department of Biomedical Science, CHA University, Seongnam, 13488, Republic of Korea.
3Department of Pathology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, 13497, Republic of Korea.
2Department of Biomedical Science, CHA University, Seongnam, 13488, Republic of Korea.
3Department of Pathology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, 13497, Republic of Korea.
†H.-R.K. and S.W. contributed equally to this work.
*Corresponding authors: correspondence to Sohyun Hwang, Hye Jin Kim or Keun-Hong Park
*Corresponding authors: correspondence to Sohyun Hwang, Hye Jin Kim or Keun-Hong Park
Abstract
While mitochondria are known to be essential for intracellular energy production and overall function, emerging evidence highlights their role in influencing cell behavior through mitochondrial transfer. This phenomenon provides a potential basis for the development of treatment strategies for tissue damage and degeneration. This study aims to evaluate whether mitochondria isolated from osteoblasts can promote osteogenic differentiation in mesenchymal stem cells (MSCs). Mitochondria from MSCs, which primarily utilize glycolysis, are compared with those from MG63 cells, which depend on oxidative phosphorylation. Mitochondria from both cell types are then encapsulated in cationic liposomes and transferred to MSCs, and their impact on differentiation is assessed. Mitochondria delivery from MG63 cells to MSCs grown in both two- and three-dimensional cultures results in increased expression of osteogenic markers, including Runt-related transcription factor 2, Osterix, and Osteopontin, and upregulation of genes involved in Bone morphogenetic protein 2 signaling and calcium import. This is accompanied by increased calcium influx and regulated by the Wnt/β-catenin signaling pathway. Transplantation of spheroids containing MSCs with MG63-derived mitochondria in bone defect animal models improves bone regeneration. The results suggest that delivery of MG63-derived mitochondria effectively guides MSCs toward osteogenesis, paving the way for the development of mitochondria-transplantation therapies.
논문정보
- Research article
- 2025년 01월 (BRIC 등록일 2025-02-03)
- 국내 연구진
- 바이오·의료융합 > 바이오·의료융합 기타
댓글 작성 로그인
팝업 닫기관련 링크
소속기관 논문보기
관련분야 논문보기
