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조종욱Jongwook Cho

GIST

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Sci. Adv., Jan 31 2025, 11 (5) eadn7577 | https://doi.org/10.1126/sciadv.adn7577 Optogenetic calcium modulation in astrocytes enhances post-stroke recovery in chronic capsular infarct

Jongwook Cho ^{1 †}, Sangkyu Lee ^{2 †}, Yeon Hee Kook ², Jiyoung Park ¹, Won Do Heo ^{3 *}, C. Justin Lee ^{2 *}, Hyoung-Ihl Kim ^{1 4 *}

¹Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
²Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34126, Republic of Korea.
³Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
⁴Department of Neurosurgery, Presbyterian Medical Center, 365 Seowon-ro, Wansan-gu, Jeonju-si, Jeollabuk-do 54987, Republic of Korea.

^†These authors contributed equally
^*Corresponding authors: correspondence to Won Do Heo, C. Justin Lee or Hyoung-Ihl Kim

Abstract

Stroke is caused by disruption of cerebral blood flow, leading to neuronal death and dysfunction in the interconnected areas, which results in a wide range of severe symptoms depending on the specific brain regions affected. While previous studies have primarily focused on direct modulation of neuronal activity for post-stroke treatment, accumulating evidence suggests that astrocytes may play a critical role in post-stroke progression and could serve as a potential therapeutic target for recovery. In this study, we investigate the effects of selective modulation of astrocytic calcium signals on chronic stroke using OptoSTIM1, an optogenetic tool that activates endogenous calcium channels. In contrast to channelrhodopsin-2 (ChR2), OptoSTIM1 robustly elevates astrocytic calcium levels, sustaining the increase for over 10 min upon a single activation. The calcium elevation in astrocytes in the ipsilesional sensory-parietal cortex leads to remarkable recovery from post-stroke impairment. Thus, manipulating intracellular calcium levels in astrocytes holds promise as a potential therapeutic strategy for improving recovery following a stroke.

논문정보

형식Research article
게재일2025년 01월 (BRIC 등록일 2025-02-19)
연구진국내 연구진
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