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Nat. Commun., 28 January 2025 | https://doi.org/10.1038/s41467-024-54676-2
Single-cell transcriptomics of bronchoalveolar lavage during PRRSV infection with different virulence
Byeonghwi Lim1,6, Seung-Chai Kim2,6, Hwan-Ju Kim2, Jae-Hwan Kim3, Young-Jun Seo1, Chiwoong Lim1, Yejee Park3, Sunirmal Sheet3, Dahye Kim3, Do-Hwan Lim4, Kyeongsoon Park5, Kyung-Tai Lee3, Won-Il Kim2 & Jun-Mo Kim1
1Functional Genomics & Bioinformatics Laboratory, Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea.
2College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do 54596, Republic of Korea.
3Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju, Jeollabuk-do 55365, Republic of Korea.
4School of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of Korea.
5Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea.
6These authors contributed equally: Byeonghwi Lim, Seung-Chai Kim.
Corresponding authors
Correspondence to Kyung-Tai Lee, Won-Il Kim or Jun-Mo Kim.
1Functional Genomics & Bioinformatics Laboratory, Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea.
2College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do 54596, Republic of Korea.
3Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju, Jeollabuk-do 55365, Republic of Korea.
4School of Systems Biomedical Science, Soongsil University, Seoul 06978, Republic of Korea.
5Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea.
6These authors contributed equally: Byeonghwi Lim, Seung-Chai Kim.
Corresponding authors
Correspondence to Kyung-Tai Lee, Won-Il Kim or Jun-Mo Kim.
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses in the global swine industry due to its high genetic diversity and different virulence levels, which complicate disease management and vaccine development. This study evaluated longitudinal changes in the immune cell composition of bronchoalveolar lavage fluid and the clinical outcomes across PRRSV strains with varying virulence, using techniques including single-cell transcriptomics. In highly virulent infection, faster viral replication results in an earlier peak lung-damage time point, marked by significant interstitial pneumonia, a significant decrease in macrophages, and an influx of lymphocytes. Viral tracking reveals less than 5% of macrophages are directly infected, and further analysis indicates bystander cell death, likely regulated by exosomal microRNAs as a significant factor. In contrast, the peak intermediate infection shows a delayed lung-damage time point with fewer cell population modifications. Furthermore, anti-inflammatory M2-like macrophages (SPP1-CXCL14high) are identified and their counts increase during the peak lung-damage time point, likely contributing to local defense and lung recovery, which is not observed in high virulent infection. These findings provide a comprehensive description of the immune cellular landscape and differential PRRSV virulence mechanisms, which will help build new hypotheses to understand PRRSV pathogenesis and other respiratory infections.
논문정보
- Research article
- 2025년 01월 (BRIC 등록일 2025-02-03)
- 국내 연구진
- 생명과학 > 생물공학
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