한빛사
>
한빛사논문
한빛사논문
- 조회112
Adv. Healthc. Mater., Jan 21 2025, | https://doi.org/10.1002/adhm.202402408
A Bioengineered Model of the Human Cornea for Preclinical Assessment of Human Ocular Exposure to Environmental Toxicants
Se-Jeong Kim 1, Ning Guo 1, Zong Yao Tan 1, Xing Gao 1, Pouria Fattahi 1, Haijiao Liu 1, Jeehan Chang 1, Mousa Younesi 1,2, Selice Jung 1, Yewhan Chung 1, Minkyung Song 1, Michelle Jung 1, Dan Dongeun Huh 1,3,4
1Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.
2Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
3NSF Science and Technology Center for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA, 19104, USA.
4Center for Innovation and Precision Dentistry, University of Pennsylvania, Philadelphia, PA, 19104, USA.
S.-j.K., N.G., and Z.Y.T. contributed equally to this work.
Corresponding Author : Dan Dongeun Huh
1Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.
2Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
3NSF Science and Technology Center for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA, 19104, USA.
4Center for Innovation and Precision Dentistry, University of Pennsylvania, Philadelphia, PA, 19104, USA.
S.-j.K., N.G., and Z.Y.T. contributed equally to this work.
Corresponding Author : Dan Dongeun Huh
Abstract
Here a bioengineered platform is introduced to investigate adverse effects of environmental materials on the human cornea. Using primary cells, this system is capable of reproducing the differentiated corneal epithelium and its underlying stroma in the human eye, which can then be treated with externally applied solid, liquid, or gaseous substances in a controlled manner and under physiologically relevant conditions. The proof-of-principle of how this system can be used to simulate human ocular exposure to different classes of environmental toxicants for direct visualization and quantitative analysis of their potential to induce acute corneal injury and inflammation is demonstrated. This model can also be further engineered to create an electromechanically actuated array of multiple human corneal tissues that can emulate spontaneous eye blinking. Using this advanced system, it is shown that blinking-like mechanical motions may play a protective role against adverse effects of environmental toxicants. This work yields an immediately deployable in vitro technology for screening ocular toxicity of existing and emerging environmental materials of various types and may enable the development of more realistic, human-relevant preclinical toxicology models complementary to traditional animal testing.
논문정보
- Research article
- 2025년 01월 (BRIC 등록일 2025-02-07)
- 국외 연구진
- 바이오·의료융합 > 의료기기
댓글 작성 로그인
팝업 닫기관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
