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한국과학기술연구원(KIST), 고려대학교

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Chem. Eng. J., 21 January 2025, 159806 | https://doi.org/10.1016/j.cej.2025.159806 Catapult-like DNA actuator for highly stable and ultrasensitive detection of urinary miRNA

Jae Chul Park ^a,1, Sungwook Park ^a,1, Seunghwan Bang ^b, Hyunro Kim^c, Yongjin Lee ^c, Min Gu Park ^e, Sung Goo Yoon ^e, Youngdo Jeong ^a,d, Hojun Kim ^a,b, Seok Ho Kang ^e, Kwan Hyi Lee ^a,b,c

^aCenter for Advanced Biomolecular Recognition, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea
^bDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), 02792, Seoul, Republic of Korea
^cKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea
^dDepartment of HY-KIST Bio-convergence, Hanyang University, Seoul 04763, Republic of Korea
^eDepartment of Urology, College of Medicine, Korea University, Seoul 02841, Republic of Korea

¹These authors contributed equally.
Corresponding authors : Hojun Kim, Seok Ho Kang, Kwan Hyi Lee

Abstract

Urinary miRNA detection is a non-invasive, simple, and user-friendly diagnostic tool, enabling frequent testing in early bladder cancer diagnosis. Field-effect transistor (FET)-based biosensors hold great promise for miRNA detection due to their exceptional sensitivity, capable of detecting single or few molecules through electrical signals. However, their practical application in urine faces significant challenges, including non-specific adsorption by abundant biomolecules and short sensing distance imposed by the Debye length in high-ionic-strength fluids like urine. Here we introduce a catapult-like actuating DNA nanostructured probe into a FET biosensor to address both non-specific adsorption and Debye length limitation issues. Notably, the designed probe effectively protects the sensor surface through steric hindrance, and when the target approaches, it activates to extend upward from the surface in a catapult-like manner. This actuation induces surface potential changes within Debye length, enabling reliable and sensitive detection of miRNA in urine. The developed biosensor achieved the detection limit of 10 fM in undiluted urine and was applied to detecting miRNA from 14 patient urine samples, including those with hematuria. It successfully discriminated bladder cancer patients from healthy donors within 20 min without RNA extraction or nucleic acid amplification.

논문정보

형식Research article
게재일2025년 01월 (BRIC 등록일 2025-01-22)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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