이나은 (광운대학교, 고려대학교) | 한빛사논문 > 한빛사

한빛사논문

조회191

이나은Na Eun Lee

광운대학교, 고려대학교

Adv. Sci., Jan 20 2025, | https://doi.org/10.1002/advs.202408771 Enhancing RT-PCR Throughput and Sensitivity through Large-Scale Sample Pooling Using a Nano-Hybrid Membrane

Na Eun Lee ^{1 2}, Kang Hyeon Kim ¹, Ji Hye Hong ^{1 3}, Seungmin Lee ^{1 3}, Jeong Soo Park ^{1 4}, Dohwan Lee ^{1 *}, Dae Sung Yoon ^{3 5 6 *}, Jeong Hoon Lee ^{1 7 *}

¹Department of Electrical Engineering, Kwangwoon University, Seoul, 01897, Republic of Korea.
²Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 02841, Republic of Korea.
³School of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea.
⁴School of Mechanical Engineering, Korea University, 145 Anam-ro, Seoungbuk-gu, Seoul, 02841, Republic of Korea.
⁵Interdisciplinary Program in Precision Public Health, Korea University, Seoul, 02841, Republic of Korea.
⁶Astrion Inc., Seoul, 02841, Republic of Korea.
⁷CALTH Inc., Changeop-ro 54, Seongnam, Gyeonggi, 13449, Republic of Korea.

^*Corresponding authors: correspondence to Dohwan Lee, Dae Sung Yoon or Jeong Hoon Lee

Abstract

During the pandemic surge, including SARS-CoV-2 and influenza, pooling samples emerged as an efficient strategy to identify infected individuals in large groups. While pooling enhances RT-PCR throughput, reducing costs and resources, it dilutes positive samples with negative ones, lowering sensitivity and increasing false negatives. This study proposes a new method to address the trade-off between pool sizes and RT-PCR accuracy. This method integrates large-scale pooling with sample enrichment using a nano-hybrid membrane, preventing the pooling-induced decrease in viral concentration and maintaining cycle threshold (Ct) values close to individual positive samples. The nano-hybrid membrane, named SIMPLE (streamlined, simple, and inexpensive method for preconcentration, lysis, and nucleic acid extraction), comprises layered red blood cell membranes, polyethersulfone, and silica membranes. Using SIMPLE, a Ct value reduction to ≈2.6 is demonstrated in pooled COVID-19 samples with a pool size of 6 and found Ct values from larger pool sizes (8, 16, 32, 64, and 128) comparable to individual positive samples.

논문정보

형식Research article
게재일2025년 01월 (BRIC 등록일 2025-01-23)
연구진국내 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기