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Trends Biotechnol., Jan 07 2025, S0167-7799(24)00321-4 | https://doi.org/10.1016/j.tibtech.2024.11.008
Acetogenesis to ethanologenesis: facilitating NADH oxidation via reductive acetate uptake
Soyoung Oh 1,2,10, Jiyeong Jeong 1,10, Byeonghyeok Park 3, Byeongchan Kang 1, Ji-Yeon Kim 1,2, Sehoon Park 4, Dong-Hun Lee 5,9, Seunghyeon Jung 4, Mungyu Lee 1,2, Wonjung Lee 6, Muhammad Yasin 7, Junhyeok Seo 2,6, Zee-Yong Park 4, Kyung-Hoon Shin 5, Volker Müller 8, In-Geol Choi 3, In Seop Chang 1,2
1School of Environment and Energy Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
2Research Center for Innovative Energy and Carbon Optimized Synthesis for Chemicals, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
3Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea
4School of Life Sciences, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
5Department of Marine Sciences and Convergent Technology, Hanyang University, Ansan 15588, Republic of Korea
6Department of Chemistry, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
7Department of Chemical Engineering, COMSATS University Islamabad, Lahore, 54000, Pakistan
8Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Frankfurt am Main, 60438 Frankfurt, Germany
9Present address: Division of Earth and Environmental System Sciences, Pukyong National University, 45, Yongso-ro, Busan 48513, Republic of Korea
10These authors contributed equally to this study
Correspondence: In-Geol Choi, In Seop Chang
1School of Environment and Energy Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
2Research Center for Innovative Energy and Carbon Optimized Synthesis for Chemicals, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
3Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea
4School of Life Sciences, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
5Department of Marine Sciences and Convergent Technology, Hanyang University, Ansan 15588, Republic of Korea
6Department of Chemistry, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea
7Department of Chemical Engineering, COMSATS University Islamabad, Lahore, 54000, Pakistan
8Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Frankfurt am Main, 60438 Frankfurt, Germany
9Present address: Division of Earth and Environmental System Sciences, Pukyong National University, 45, Yongso-ro, Busan 48513, Republic of Korea
10These authors contributed equally to this study
Correspondence: In-Geol Choi, In Seop Chang
Abstract
(Homo)acetogens, including Clostridium spp., represent an enigma in metabolic flexibility and diversity. Eubacterium callanderi KIST612 is an acetogen that produces n-butyrate with carbon monoxide (CO) as the carbon and energy source; however, the production route is unknown. Here, we report that its distinctive butyrate formation links to reductive acetate uptake, suggesting that acetate (the end-product) is reuptake, leading to a physiological advantage through NADH oxidation. Thus, we introduced an ethanol production pathway from acetyl-CoA as a competitive pathway for butyrate production. Consequently, the metabolic pathway in our mutants switched from acetogenesis to 'ethanologenesis', eliminating butyrate production and the uptake of previously produced acetate. The metabolic shifts occurred toward greater NADH oxidation, facilitating CO oxidation and productivity, which is a survival mechanism at the thermodynamic edge. This metabolic shift to a single product holds potential to revolutionize product separation strategies in synthetic gas (syngas)-based biorefineries.
논문정보
- Research article
- 2025년 01월 (BRIC 등록일 2025-01-14)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오소재
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