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류광민Kwangmin Ryu
KAIST
Nucleic Acids Res., Jan 07 2025, 53 (1) gkae1281 | https://doi.org/10.1093/nar/gkae1281
Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF
Hyoungjoon Ahn1,†, Jeongmin Yu1,†, Kwangmin Ryu1,†, Jaeseung Ryu1, Sera Kim1, Jae Yeong Park1, Ji Kwang Kim1, Inhong Jung1, Haejin An1, Sehoon Hong1, Eunha Kim1, Kihyun Park1, Myunghwan Ahn2, Sunwoo Min1, Inkyung Jung1, Daeyoup Lee1, Thomas Lee3, Youngjoo Byun2, Ji-Joon Song1, Jaehoon Kim1, Won-Ki Cho1,*, Gwangrog Lee1,* and Seyun Kim1,4,5,*
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
2College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea
3Department of Biochemistry, University of Colorado, 3415 Colorado Avenue, Boulder 80303, USA
4KAIST Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
5KAIST Stem Cell Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
*To whom correspondence should be addressed. : Seyun Kim
Correspondence may also be addressed to Gwangrog Lee.
Correspondence may also be addressed to Won-Ki Cho.
Present addresses:
Eunha Kim, Department of Neuroscience, Korea University College of Medicine, Seoul 02841, Republic of Korea.
Sunwoo Min, Department of Biochemistry, Chungnam National University, Daejeon 34134, Republic of Korea.
†The first three authors should be regarded as Joint First Authors.
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
2College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea
3Department of Biochemistry, University of Colorado, 3415 Colorado Avenue, Boulder 80303, USA
4KAIST Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
5KAIST Stem Cell Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea
*To whom correspondence should be addressed. : Seyun Kim
Correspondence may also be addressed to Gwangrog Lee.
Correspondence may also be addressed to Won-Ki Cho.
Present addresses:
Eunha Kim, Department of Neuroscience, Korea University College of Medicine, Seoul 02841, Republic of Korea.
Sunwoo Min, Department of Biochemistry, Chungnam National University, Daejeon 34134, Republic of Korea.
†The first three authors should be regarded as Joint First Authors.
Abstract
Serum response factor (SRF) is a master transcription factor that regulates immediate early genes and cytoskeletal remodeling genes. Despite its importance, the mechanisms through which SRF stably associates with its cognate promoter remain unknown. Our biochemical and protein-induced fluorescence enhancement analyses showed that the binding of SRF to serum response element was significantly increased by inositol polyphosphate multikinase (IPMK), an SRF cofactor. Moreover, real-time tracking of SRF loci in live cell nuclei demonstrated that the chromatin residence time of SRF was reduced by IPMK depletion in fibroblasts. Conversely, elevated IPMK levels extended the SRF-chromatin association. We identified that IPMK binds to the intrinsically disordered region of SRF, which is required for the IPMK-induced stable interaction of SRF with DNA. IPMK-mediated conformational changes in SRF were observed by single-molecule fluorescence resonance energy transfer assays. Therefore, our findings demonstrate that IPMK is a critical factor for promoting high-affinity SRF-chromatin association and provide insights into the mechanisms of SRF-dependent transcription control via chaperone-like activity.
논문정보
- Research article
- 2025년 01월 (BRIC 등록일 2025-01-09)
- 국내(교신)+국외 연구진
- 생명과학 > 생화학
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