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Nucleic Acids Res., Jan 07 2025, 53 (1) gkae1292 | https://doi.org/10.1093/nar/gkae1292 Quadruplet codon decoding-based versatile genetic biocontainment system

Yun-Nam Choi^1,†, Donghyeon Kim^1,†, Seongbeom Lee¹, Ye Rim Shin¹ and Jeong Wook Lee^1,2,*

¹Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
²School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea

^*To whom correspondence should be addressed.
^†The first two authors should be regarded as Joint First Authors.

Abstract

Biological resources, such as sequence information, genetic traits, materials and strains, pose risks when inadvertently released or deliberately misused. To address these concerns, we developed Quadruplet COdon DEcoding (QCODE), a versatile genetic biocontainment strategy that introduces a quadruplet codon (Q-codon) causing frameshifts, hindering proper gene expression. Strategically incorporating Q-codons in multiple genes prevents genetic trait escape, unallowed proliferation of microbial strains and unauthorized leakages of genetic materials. This multifaceted strategy, integrating Q-codons for genetic traits, materials and strains, ensures robust biocontainment across various levels. Notably, our system maintains sequence protection, safeguarding genetic sequence information against unauthorized access. The QCODE approach offers a versatile, efficient and compact solution to enhance biosecurity in diverse biological research settings.

논문정보

형식Research article
게재일2025년 01월 (BRIC 등록일 2025-01-10)
연구진국내 연구진
분야 생명과학 > 생물공학

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