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조회138

Wenzhou Medical University

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J. Control. Release, Dec 31 2024, 378 S0168-3659(24)00916-7 | https://doi.org/10.1016/j.jconrel.2024.12.061 Hyperglycemia-responsive nitric oxide-releasing biohybrid cryogels with cascade enzyme catalysis for enhanced healing of infected diabetic wounds

Jiajun Hu^a,b,c, Kairui Duan ^a,b, Yueming Zhao ^a,b, Hangbin Xv ^c, Xinxin Ge ^c, Mian Lin ^a,b, Hu Zhu ^a,b, Dingze Chen ^c, Hui Deng ^c, Bae Hoon Lee ^a,b,c

^aJoint Research Center of Medicine, The Affiliated Xiangshan Hospital of Wenzhou Medical University, Ningbo, Zhejiang 315700, China
^bZhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325011, China
^cDepartment of Periodontics, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China

Corresponding authors: Hui Deng, Bae Hoon Lee

Abstract

Diabetic wound infections are a frequent complication for diabetic patients, and conventional treatment for combating diabetic wound infections relies on antibiotics. However, the misuse and overuse of antibiotics have led to the emergence of drug-resistant bacteria, making these infections challenging to treat. Thus, there is an urgent need for alternative strategies to effectively manage diabetic wound infections. Herein, we have developed a hyperglycemia-responsive antibacterial cryogel system that can generate and release hydrogen peroxide (H₂O₂) and nitric oxide (NO). This system involves incorporating glucose oxidase (GO) and L-Arginine (L-Arg: A) into hyaluronic acid aldehyde methacryloyl (HAAMA: H) and gelatin methacryloyl (GelMA: G) hybrid cryogels (GOA@HG). HAAMA facilitated higher loading and longer stability of L-Arg and GO via a Schiff base reaction. In vitro studies demonstrate that GOA@HG cryogels exhibited outstanding breathability, effective exudate management, and excellent hemostasis capabilities. Moreover, this system could consume excess glucose in diabetic wounds and efficiently eliminate bacteria through the cascaded release of H₂O₂ and NO without causing antibiotic resistance. In vivo studies further reveal that GOA@HG cryogels significantly enhanced the healing of infected diabetic wounds by inhibiting bacterial growth, accelerating blood vessel formation, and promoting collagen deposition. Overall, GOA@HG cryogels displayed remarkable wound dressing properties and synergistic antimicrobial effects owing to glucose-responsive H₂O₂ and NO release, which could serve as a highly efficient therapeutic strategy for treating infected diabetic wounds.

논문정보

형식Research article
게재일2024년 12월 (BRIC 등록일 2025-01-13)
연구진국외 연구진
분야 바이오·의료융합 > 바이오소재

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