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ACS Nano, Dec 23 2024, | https://doi.org/10.1021/acsnano.4c12364
Clinically-Driven Rapidly Developed Nanoparticle Corona for Label-Free Cerebrospinal Fluid Leakage Detection
Seungju Lee 1, Gwanghui Ryu 2, Seyoung Shin 1, Woojin Kim 3, Minyeong Yoon 1, Yeji Kim 4, Seongjun Park 5,6, YongJoo Kim 7, Soo-Yeon Cho 1
1School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
2Department of Otorhinolaryngology-Head and Neck Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea.
3Department of Materials Science and Engineering, Kookmin University, Seoul 02707, Republic of Korea.
4Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
5College of Transdisciplinary Innovations, Seoul National University, Seoul 08826, Republic of Korea.
6Department of Biomedical Sciences, Seoul National University, Seoul 03080, Republic of Korea.
7Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
S.L. and G.R. contributed equally to this work.
Corresponding Author : Soo-Yeon Cho
1School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
2Department of Otorhinolaryngology-Head and Neck Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea.
3Department of Materials Science and Engineering, Kookmin University, Seoul 02707, Republic of Korea.
4Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
5College of Transdisciplinary Innovations, Seoul National University, Seoul 08826, Republic of Korea.
6Department of Biomedical Sciences, Seoul National University, Seoul 03080, Republic of Korea.
7Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.
S.L. and G.R. contributed equally to this work.
Corresponding Author : Soo-Yeon Cho
Abstract
Rapid diagnosis of cerebrospinal fluid (CSF) leaks is critical as endoscopic endonasal skull base surgery gains global prominence. Current clinical methods such as endoscopic examination with and without intrathecal injection of fluorescent dye are invasive and rely on subjective judgment by physicians, highlighting the clinical need for label-free point-of-care (POC). However, a viable solution remains undeveloped due to the molecular complexity of CSF rhinorrhea mixed with nasal discharge and the scarcity of specific biomarkers, delaying sensor development. In this study, we accelerated the development of a label-free CSF detection method for clinical use using a nanoparticle corona. We engineered corona nanointerfaces on near-infrared (nIR) fluorescent single-walled carbon nanotubes (SWCNTs) through noncovalent functionalization with 12 custom-designed poly(ethylene glycol) (PEG) lipids. By high-throughput screening of the corona library for the CSF biomarker β-trace protein (βTP), we selected the optimal corona, achieving a limit of detection (LOD) down to 1.46 mg/L, maintaining its selectivity even in human nasal discharge. Using molecular dynamics and docking simulations, we characterized the 3D morphology and βTP binding energy of the optimal corona in a quantified way. The corona nanosensor accurately diagnosed CSF leakages from eight patients having lumbar drainage and one patient with CSF leakage due to diverse diseases without any sample preparations. By integrating the nanosensor with custom-designed in vivo and in vitro form factors such as a camera and endoscope, we showed its potential for versatile and practical use in clinical settings. This accelerated sensor development platform can meet future urgent clinical demands for various diseases and conditions.
논문정보
- Research article
- 2024년 12월 (BRIC 등록일 2024-12-24)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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