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조회155

이영윤Young-Yoon Lee

서울대학교

Nat. Rev. Mol. Cell Biol., 19 December 2024 | https://doi.org/10.1038/s41580-024-00805-0 The biogenesis and regulation of animal microRNAs

Haedong Kim ^1,2,3,4, Young-Yoon Lee ^1,2,4 & V. Narry Kim^1,2,*

¹Center for RNA Research, Institute for Basic Science, Seoul, Republic of Korea.
²School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
³Present address: Department of Genome Sciences, University of Washington, Seattle, WA, USA.
⁴These authors contributed equally: Haedong Kim, Young-Yoon Lee.

^*Corresponding author: correspondence to V. Narry Kim

Abstract

MicroRNAs (miRNAs) are small, yet profoundly influential, non-coding RNAs that base-pair with mRNAs to induce RNA silencing. Although the basic principles of miRNA biogenesis and function have been established, recent breakthroughs have yielded important new insights into the molecular mechanisms of miRNA biogenesis. In this Review, we discuss the metazoan miRNA biogenesis pathway step-by-step, focusing on the key biogenesis machinery, including the Drosha–DGCR8 complex (Microprocessor), exportin-5, Dicer and Argonaute. We also highlight newly identified cis-acting elements and their impact on miRNA maturation, informed by advanced high-throughput and structural studies, and discuss recently discovered mechanisms of clustered miRNA processing, target recognition and target-directed miRNA decay (TDMD). Lastly, we explore multiple regulatory layers of miRNA biogenesis, mediated by RNA–protein interactions, miRNA tailing (uridylation or adenylation) and RNA modifications.

논문정보

형식Review Paper
게재일2024년 12월 (BRIC 등록일 2024-12-24)
연구진국내 연구진
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