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순천대학교 약학대학

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ACS Central Sci., December 16, 2024 | https://doi.org/10.1021/acscentsci.4c01822 A Pair of Fluorescent Probes Enabling Precise Diagnosis of Liver Cancer by Complementary Imaging

Min Gao,^1* Sun Hyeok Lee², Haw-Young Kwon³, Larissa Miasiro Ciaramicoli³, Eunsol Jo⁴, Young Hyun Yu⁴, Fengming Li⁵, Beomsue Kim⁶, Kyungtae Hong⁷, Jun-Seok Lee⁸, Namhui Kim⁹, Yoojin Oh⁹, Chun Young Im⁹, Chris Soon Heng Tan,^5* Hyung-Ho Ha,^4* and Young-Tae Chang^3*

¹School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China
²School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
³Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
⁴College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, Sunchon 57922, Republic of Korea
⁵Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, P. R. China
⁶Neural Circuits Research Group, Korea Brain Research Institute, Daegu 41062, Republic of Korea
⁷Bio-Med Program, KIST-School UST, Biomedical Research Division, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea
⁸Department of Pharmacology, Korea University, Seoul 02841, Republic of Korea
⁹New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation(K-MEDIhub), Daegu 41061, Republic of Korea

^*Corresponding Authors : Min Gao, Soon Heng Tan, Hyung-Ho Ha, and Young-Tae Chang

Abstract

Hepatocellular carcinoma (HCC) is by far the predominant malignant liver cancer, with both high morbidity and mortality. Early diagnosis and surgical resections are imperative for improving the survival of HCC patients. However, limited by clinical diagnosis methods, it is difficult to accurately distinguish tumor tissue and its boundaries in the early stages of cancer. Herein, we report two fluorescent probes, cLG and hLR, for the detection of cancer and healthy cells, respectively, enabling the precise diagnosis of liver cancer by providing complementary imaging. These two fluorescent probes could selectively stain the target cells in the liver tissue imaging, which is confirmed by H&E and antibody staining. Moreover, for the first time, the cancerous area and healthy area are clearly identified by the cocktail of these two probes, suggesting its potential to be used in fluorescence-guided surgery. Finally, we identify transporter SLC27A2 as the gating target of cLG through a systematic transporter screen using a CRISPR activation library. SMPD1 was identified as the target of hLR through a thermal proteome profiling. Therefore, the development of these two highly specific probes offers complementary imaging and provides a unique diagnostic tool for cancer disease, even for fluorescence-guided surgery.

논문정보

형식Research article
게재일2024년 12월 (BRIC 등록일 2024-12-18)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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