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Adv. Healthc. Mater., Dec 15 2024, | https://doi.org/10.1002/adhm.202403192
Radiation-Resistant Bacteria Deinococcus radiodurans-Derived Extracellular Vesicles as Potential Radioprotectors
Jeong Moo Han 1,2,3,4, Godfrey Mwiti 5,6, Seo-Joon Yeom 1,7, Jaeyoon Lim 1,6, Woo Sik Kim 8, Sangyong Lim 1,9, Seung-Taik Lim 2, Eui-Baek Byun 1
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
2Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
3Department of Microbiology and Immunology, College of Medicine, Seoul National University, Seoul, 03080, South Korea.
4Institute for Data Innovation in Science, Seoul National University, Seoul, 08826, South Korea.
5Department of Microbiology and Immunology, The University of Melbourne, Melbourne, 3000, Australia.
6Department of Food and Nutrition, Chungnam National University, Daejeon, 34134, Republic of Korea.
7Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, Republic of Korea.
8Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
9Department of Radiation Science, University of Science and Technology, Daejeon, 01812, Republic of Korea.
Corresponding Author: Eui-Baek Byun
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
2Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
3Department of Microbiology and Immunology, College of Medicine, Seoul National University, Seoul, 03080, South Korea.
4Institute for Data Innovation in Science, Seoul National University, Seoul, 08826, South Korea.
5Department of Microbiology and Immunology, The University of Melbourne, Melbourne, 3000, Australia.
6Department of Food and Nutrition, Chungnam National University, Daejeon, 34134, Republic of Korea.
7Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, Republic of Korea.
8Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
9Department of Radiation Science, University of Science and Technology, Daejeon, 01812, Republic of Korea.
Corresponding Author: Eui-Baek Byun
Abstract
The increasing use of radiation presents a risk of radiation exposure, making the development of radioprotectors necessary. In the previous study, it is investigated that Deinococcus radiodurans (R1-EVs) exert the antioxidative properties. However, the radioprotective activity of R1-EVs remains unclear. In the present study, the protective effects of R1-EVs against total body irradiation (TBI)-induced acute radiation syndrome (ARS) are investigated. To assess R1-EVs' radioprotective efficacy, ARS is induced in mice with 8 Gy of TBI, and protection against hematopoietic (H)- and gastrointestinal (GI)-ARS is evaluated. The survival rate of irradiated mice group decreases substantially after irradiation. In contrast, pretreatment with R1-EVs increases the survival rates of the mice. The administration of R1-EVs provides effective protection against radiation-induced death of bone marrow cells and splenocytes by scavenging reactive oxygen species (ROS). Additionally, R1-EVs protect both intestinal stem and epithelial cells from radiation-induced apoptosis. R1-EVs stimulate the production of short-chain fatty acids in the gastrointestinal tract, suppress proinflammatory cytokines, and increase regulatory T cells in pretreated mice versus the irradiation-only group. Proteomic analysis shows that the R1-EV proteome is significantly enriched with proteins involved in oxidative stress response. These findings highlight R1-EVs as potent radioprotectors with applications against radiation damage and ROS-mediated diseases.
논문정보
- Research article
- 2024년 12월 (BRIC 등록일 2024-12-17)
- 국내(교신)+국외 연구진
- 생명과학 > 미생물학
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