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Proc. Natl. Acad. Sci. USA, Dec 03 2024, 121 (49) | https://doi.org/10.1073/pnas.2411286121
Chronologically inappropriate morphogenesis (Chinmo) is required for maintenance of larval stages of fall armyworm
Xien Chen1,2, Jinmo Koo2,3, Surjeet Kumar Arya, and Subba Reddy Palli4
Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY 40546
1Present address: Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
2X.C. and J.K. contributed equally to this work.
3Present address: Department of Entomology, University of California, Riverside, CA 92521
4To whom correspondence may be addressed. : Subba Reddy Palli
Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY 40546
1Present address: Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
2X.C. and J.K. contributed equally to this work.
3Present address: Department of Entomology, University of California, Riverside, CA 92521
4To whom correspondence may be addressed. : Subba Reddy Palli
Abstract
Broad complex (Br-C) and eip93F (E93) transcription factors promote insect metamorphosis from larva to pupa and from pupa to adult, respectively. Recently, chronologically inappropriate morphogenesis (Chinmo) has been proposed as a larval specifier in Drosophila melanogaster. However, whether Chinmo is required for larval maintenance in lepidopteran insects, the underlying mechanisms involved in maintaining the larval stage, and its interactions with the JH signaling pathway are not well understood. Here, we used a binary transgenic CRISPR/Cas9 system to knockout Chinmo and Kr-h1 (primary response gene in the JH signaling pathway) in the fall armyworm (FAW). Kr-h1 knockout induced premature metamorphosis only after L5 (penultimate), whereas Chinmo and Kr-h1 double knockout induced premature metamorphosis in L3. Sequencing and differential gene expression (DEG) analysis of RNA isolated from mutants and single-cell multiome ATAC analysis of Chinmo, Kr-h1, and Chinmo and Kr-h1 double knockout Sf9 cells revealed that Chinmo participates in chromatin modifications that prevent the promoter accessibility and expression of metamorphosis promoting genes. These results suggest that Chinmo is a larval specifier that plays a major role in preventing metamorphosis in early larval stages by controlling chromatin accessibility near the promoters of genes such as Br-C and E93 required for pupal and adult development.
논문정보
- Research article
- 2024년 12월 (BRIC 등록일 2024-11-27)
- 국외 연구진
- 생명과학 > 응용생물화학
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