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Longlong LiLonglong Li
전남대학교
ACS Nano, Nov 26 2024, | https://doi.org/10.1021/acsnano.4c05365
Graphene SU-8 Platform for Enhanced Cardiomyocyte Maturation and Intercellular Communication in Cardiac Drug Screening
Longlong Li 1, Arunkumar Shanmugasundaram 1,2, Jongyun Kim 1,2,3, Nomin-Erdene Oyunbaatar 1,2, Pooja P Kanade 4, Seong-Eung Cha 5, Daeyun Lim 6, Chil-Hyoung Lee 6, Young-Baek Kim 6, Bong-Kee Lee 1, Eung-Sam Kim 5, Dong-Weon Lee 1,2,3
1Department of Mechanical Engineering, Chonnam National University, Gwangju 61186, Republic of Korea.
2Advanced Medical Device Research Center for Cardiovascular Disease, Chonnam National University, Gwangju 61186, Republic of Korea.
3Center for Next-Generation Sensor Research and Development, Chonnam National University, Gwangju 61186, Republic of Korea.
4Centre for Quantum Materials and Technology, School of Mathematics and Physics, Queen's University Belfast, Northern Ireland, Belfast BT7 1NN, U.K.
5Department of Biological Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
6Green Energy & Nano Technology R&D Group, Korea Institute of Industrial Technology, Gwangju 61012, Republic of Korea.
Corresponding Author : Dong-Weon Lee
1Department of Mechanical Engineering, Chonnam National University, Gwangju 61186, Republic of Korea.
2Advanced Medical Device Research Center for Cardiovascular Disease, Chonnam National University, Gwangju 61186, Republic of Korea.
3Center for Next-Generation Sensor Research and Development, Chonnam National University, Gwangju 61186, Republic of Korea.
4Centre for Quantum Materials and Technology, School of Mathematics and Physics, Queen's University Belfast, Northern Ireland, Belfast BT7 1NN, U.K.
5Department of Biological Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
6Green Energy & Nano Technology R&D Group, Korea Institute of Industrial Technology, Gwangju 61012, Republic of Korea.
Corresponding Author : Dong-Weon Lee
Abstract
Cell culture substrates designed for myocardial applications are pivotal in promoting the maturation and functional integration of cardiomyocytes. However, traditional in vitro models often inadequately mimic the diverse biochemical signals and electrophysiological properties of mature cardiomyocytes. Herein, we propose the application of monolayer graphene, transferred onto SU-8 cantilevers integrated with a microelectrode array, to evaluate its influence on the structural, functional, and electro-mechano-physiological properties of cardiomyocytes. The monolayer graphene, prepared using chemical vapor deposition, is adeptly transferred to the target substrates via thermal release tape. The electrical conductivity of these graphene-enhanced SU-8 substrates is about 1600 S/cm, markedly surpassing that of previously reported cell culture substrates. Immunofluorescence staining and Western blot analyses reveal that the electrically conductive graphene significantly enhances cardiomyocyte maturation and cardiac marker expression compared to bare SU-8 substrates. Cardiomyocytes cultured on graphene-transferred substrates exhibit conduction velocity approximately 3.4 times greater than that of the control group. Such improvements in cardiac marker expression, mechano-electrophysiological performance lead to better responsiveness to cardiovascular drugs, such as Verapamil and Isoproterenol. While the graphene monolayer does not fully replicate the complex environment found in native cardiac tissue, its use on SU-8 substrates offers a feasible approach for accelerating cardiomyocyte maturation and facilitating drug screening applications.
논문정보
- Research article
- 2024년 11월 (BRIC 등록일 2024-11-29)
- 국내(교신)+국외 연구진
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