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Proc. Natl. Acad. Sci. USA, Dec 03 2024, 121 (49) | https://doi.org/10.1073/pnas.2407524121
Spatiotemporal bifurcation of HY5-mediated blue-light signaling regulates wood development during secondary growth
Hyeona Hwanga, Yookyung Lima, Myung-Min Ohb, Hyunmo Choic, Donghwan Shimd, Young Hun Songe, and Hyunwoo Choa,1
aDepartment of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea
bDepartment of Horticultural Science, Chungbuk National University, Cheongju 28644, Korea
cDepartment of Forest Bioresources, National Institute of Forest Science, Suwon 16631, Korea
dDepartment of Biological Sciences, Chungnam National University, Daejeon 34134, Korea
eDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea
1To whom correspondence may be addressed. : Hyunwoo Cho
aDepartment of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea
bDepartment of Horticultural Science, Chungbuk National University, Cheongju 28644, Korea
cDepartment of Forest Bioresources, National Institute of Forest Science, Suwon 16631, Korea
dDepartment of Biological Sciences, Chungnam National University, Daejeon 34134, Korea
eDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea
1To whom correspondence may be addressed. : Hyunwoo Cho
Abstract
Plants have evolved photoreceptors to optimize their development during primary growth, including germination, hypocotyl elongation, cotyledon opening, and root growth, allowing them to adapt to challenging light conditions. The light signaling transduction pathway during seedling establishment has been extensively studied, but little molecular evidence is available for light-regulated secondary growth, and how light regulates cambium-derived tissue production remains largely unexplored. Here, we show that CRYPTOCHROME (CRY)-dependent blue light signaling and the subsequent attenuation of ELONGATED HYPOCOTYL 5 (HY5) movement to hypocotyls are key inducers of xylem fiber differentiation in Arabidopsis thaliana. Using grafted chimeric plants and hypocotyl-specific transcriptome sequencing of light signaling mutants under controlled light conditions, we demonstrate that the perception of blue light by CRYs in shoots drives secondary cell wall (SCW) deposition at xylem fiber cells during the secondary growth of hypocotyls. We propose that HY5 is a blue light-responsive mobile protein that inhibits xylem fiber formation via direct transcriptional repression of NAC SECONDARY WALL THICKENING PROMOTING 3 (NST3). CRYs retain HY5 in the nucleus, impede its long-distance transport from leaf to hypocotyl, and they initiate NST3-driven SCW gene expression, thereby triggering xylem fiber production. Our findings shed light on the long-range CRYs-HY5-NST3 signaling cascade that shapes xylem fiber development, highlighting the activity of HY5 as a transcriptional repressor during secondary growth.
논문정보
- Research article
- 2024년 12월 (BRIC 등록일 2024-11-28)
- 국내 연구진
- 생명과학 > 식물학
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