한빛사논문
- 조회550
Eun Seon Chung 1,6, Prathitha Kar 2,3,6, Maliwan Kamkaew 1, Ariel Amir 4 & Bree B. Aldridge 1,5
1Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Stuart B. Levy Center for Integrated Management of Antimicrobial Resistance, Boston, MA, USA.
2John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
3Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
4Department of Complex Systems, Weizmann Institute of Science, Rehovot, Israel.
5Department of Biomedical Engineering, Tufts University School of Engineering, Medford, MA, USA.
6These authors contributed equally: Eun Seon Chung, Prathitha Kar.
*Corresponding author: correspondence to Ariel Amir or Bree B. Aldridge
Abstract
Difficulties in antibiotic treatment of Mycobacterium tuberculosis (Mtb) are partly thought to be due to heterogeneity in growth. Although the ability of bacterial pathogens to regulate growth is crucial to control homeostasis, virulence and drug responses, single-cell growth and cell cycle behaviours of Mtb are poorly characterized. Here we use time-lapse, single-cell imaging of Mtb coupled with mathematical modelling to observe asymmetric growth and heterogeneity in cell size, interdivision time and elongation speed. We find that, contrary to Mycobacterium smegmatis, Mtb initiates cell growth not only from the old pole but also from new poles or both poles. Whereas most organisms grow exponentially at the single-cell level, Mtb has a linear growth mode. Our data show that the growth behaviour of Mtb diverges from that of model bacteria, provide details into how Mtb grows and creates heterogeneity and suggest that growth regulation may also diverge from that in other bacteria.
논문정보
- Research article
- 2024년 11월 (BRIC 등록일 2024-11-19)
- 국외 연구진
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