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조회312

성균관대학교, IBS

Nano Converg., Oct 31 2024, 11 (1) 44 | https://doi.org/10.1186/s40580-024-00451-4 Simple and Cost-Effective Generation of 3D Cell Sheets and Spheroids Using Curvature-Controlled Paraffin Wax Substrates

Huijung Kim^1,2,3, Kyeong‑Mo Koo^1,2, Chang‑Dae Kim^1,2, Min Ji Byun^1,2, Chun Gwon Park^1,2, Hyungbin Son⁴, Hyung‑Ryong Kim^5* and Tae‑Hyung Kim^1,2*

¹Department of Biomedical Engineering, Institute for Cross‑Disciplinary Studies (ICS), Sungkyunkwan University (SKKU), Suwon 16419, Gyeonggi, Republic of Korea

²Department of Intelligent Precision Healthcare Convergence, ICS, SKKU, Suwon 16419, Gyeonggi, Republic of Korea

³Center for Neuroscience Imaging Research (CNIR), Institute for Basic Science (IBS), Suwon 16419, Republic of Korea

⁴School of Integrative Engineering, Chung-Ang University, 84 Heukseuk‑Ro, Dongjak‑Gu, Seoul 06974, Republic of Korea

⁵Department of Pharmacology, College of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of Korea

*Corresponding authors

Correspondence to Hyung-Ryong Kim or Tae-Hyung Kim.

Abstract

The challenges associated with animal testing in pharmaceutical development have driven the search for alternative in vitro models that mimic human tissues more accurately. In this study, we present a simple and cost-effective method for generating 3D cell sheets and spheroids using curvature-controlled paraffin wax films, which are easily accessible laboratory materials that eliminate the need for extracellular matrix (ECM) components or thermo-responsive polymers. By adjusting the curvature of the paraffin wax film, we successfully generated human periodontal ligament fibroblast (HPdLF) cell sheets and bone marrow-derived mesenchymal stem cell (hBMSC) spheroids. Key parameters, such as cell density, substrate curvature, and incubation time, were identified as critical factors for optimizing the formation of these 3D structures. In addition, the use of quantum dots (QDs) for cell tracking enabled long-term visualization and distinction between different cell types within complex tissue-like structures. We further demonstrated that wrapping the hBMSC spheroids with HPdLF cell sheets partially replicated the connective tissue structure of the periodontal ligament surrounding the tooth root. This highlights the potential of this platform for the construction of more sophisticated tissue-mimicking assemblies. In conclusion, curvature-controlled paraffin wax films provide a versatile and practical approach for 3D cell cultures. This simplifies the generation of both cell sheets and spheroids, offering a promising tool for tissue engineering and regenerative medicine applications, where precise cell-to-cell interactions are essential.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-11-11)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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