한빛사논문
Dohui Kim 1,6, Hyeonji Lim 2,6, Jaeseung Youn 1, Tae-Eun Park 2,7,* & Dong Sung Kim 1,3,4,5,7,*
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, South Korea.
2Department of Biomedical Engineering, College of Information and Biotechnology, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea.
3Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, South Korea.
4School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, South Korea.
5Institute for Convergence Research and Education in Advanced Technology, Yonsei University, Seoul, South Korea.
6These authors contributed equally: Dohui Kim, Hyeonji Lim.
7These authors jointly supervised this work: Dong Sung Kim, Tae-Eun Park
*Corresponding authors: correspondence to Tae-Eun Park or Dong Sung Kim
Abstract
The application of organoids has been limited by the lack of methods for producing uniformly mature organoids at scale. This study introduces an organoid culture platform, called UniMat, which addresses the challenges of uniformity and maturity simultaneously. UniMat is designed to not only ensure consistent organoid growth but also facilitate an unrestricted supply of soluble factors by a 3D geometrically-engineered, permeable membrane-based platform. Using UniMat, we demonstrate the scalable generation of kidney organoids with enhanced uniformity in both structure and function compared to conventional methods. Notably, kidney organoids within UniMat show improved maturation, showing increased expression of nephron transcripts, more in vivo-like cell-type balance, enhanced vascularization, and better long-term stability. Moreover, UniMat’s design offers a more standardized organoid model for disease modeling and drug testing, as demonstrated by polycystic-kidney disease and acute kidney injury modeling. In essence, UniMat presents a valuable platform for organoid technology, with potential applications in organ development, disease modeling, and drug screening.
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