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Nano Converg., Oct 24 2024, 11 (1) | https://doi.org/10.1186/s40580-024-00449-y Highly sensitive multiplexed colorimetric lateral flow immunoassay by plasmon-controlled metal-silica isoform nanocomposites: PINs

Minsup Shin^1†, Wooyeon Kim^1†, Kwanghee Yoo¹, Hye‑Seong Cho¹, Sohyeon Jang¹, Han‑Joo Bae¹, Jaehyun An², Jong‑chan Lee³, Hyejin Chang⁴, Dong‑Eun Kim¹, Jaehi Kim^1*, Luke P. Lee^5,6,7* and Bong‑Hyun Jun^1*

¹Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.

²Company of BioSquare, Hwaseong 18449, Republic of Korea.

³Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.

⁴Division of Science Education, Kangwon National University, Chuncheon 24341, Republic of Korea.

⁵Renal Division and Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA.

⁶Department of Bioengineering, Department of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, CA 94720, USA.

⁷Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon 16419, Republic of Korea.

†Minsup Shin and Wooyeon Kim have contributed equally to this work.

*Correspondence: Jaehi Kim, Luke P. Lee, Bong‑Hyun Jun

Abstract

Lateral flow assay (LFA) systems use metal nanoparticles for rapid and convenient target detection and are extensively studied for the diagnostics of various diseases. Gold nanoparticles (AuNPs) are often used as probes in LFAs, displaying a single red color. However, there is a high demand for colorimetric LFAs to detect multiple biomarkers, requiring the use of multicolored NPs. Here, we present a highly sensitive multiplexed colorimetric lateral flow immunoassay by multicolored Plasmon-controlled metal-silica Isoform Nanocomposites (PINs). We utilized the localized surface plasmon resonance effect to create multi-colored PINs by precisely adjusting the distance between the NPs on the surface of PINs through the controlled addition of reduced gold and silver precursors. Through simulations, we also confirmed that the distance between nanoparticles on the surface of PINs significantly affects the color and colorimetric signal intensity of the PINs. We achieved multicolored PINs that exhibit stronger colorimetric signals, offering a new solution for LFA detection with high sensitivity and a 33 times reduced limit of detection (LOD) while maintaining consistent size deviations within 5%. We expect that our PINs-based colorimetric LFA will facilitate the sensitive and simultaneous detection of multiple biomarkers in point-of-care testing.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-10-25)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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