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Nat. Commun., Oct 23 2024, 15 (1) | https://doi.org/10.1038/s41467-024-53246-w Oxygenating respiratoid biosystem for therapeutic cell transplantation

Seonmi Jang^1,6, Chaerim Yoo^1,6, Hyung Shik Kim^1,2, Jiyun Kim¹ & Dong Yun Lee^1,3,4,5

¹Department of Bioengineering, College of Engineering, and BK FOUR Biopharmaceutical Innovation Leader for Education and Research Group, Hanyang University, Seoul, Republic of Korea.

²Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA, USA.

³Institute of Nano Science and Technology (INST), Hanyang University, Seoul, Republic of Korea.

⁴Institute for Bioengineering and Biopharmaceutical Research (IBBR), Hanyang University, Seoul, Republic of Korea.

⁵Elixir Pharmatech Inc, Seoul, Republic of Korea.

⁶These authors contributed equally: Seonmi Jang, Chaerim Yoo.

Corresponding author

Correspondence to Dong Yun Lee.

Abstract

In this study, we address the persistent challenge of providing adequate oxygen to transplanted cells by introducing a respiratoid biosystem. Central to our strategy is the chloroplast-transit-peptide (CTP), crucial for optimal oxygenation. Through conjugation of CTP with alginate, we achieve stabilization of chloroplast structure. Strategically anchored to the outer chloroplast membrane, CTP not only ensures structural integrity but also upregulates key photosynthesis-associated genes. This biosystem demonstrates exceptional efficacy in spontaneously generating oxygen, particularly under hypoxic conditions (~1% pO₂). In an application, pancreatic islets encapsulated within the respiratoid biosystem and intraperitoneally implanted in diabetic mice maintain normal glucose levels effectively. Insulin secretion persists for 100 days post-xenotransplantation without the need for immunosuppressant administration, highlighting the reliance on the respiratoid biosystem's oxygen supply and structural stability. Our study demonstrates the respiratoid biosystem as a platform in tissue engineering, offering a nature-inspired solution to the critical challenge of spontaneous oxygen supply.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-10-28)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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