한빛사논문
Choongman Lee,1,2 Andrea Quintana,1,2 Ida Suppanz,1,3 Alejandro Gomez-Auli,1,3 Gerhard Mittler,1,3 and Ibrahim I. Cisse´ 1,2,4,*
1Max Planck Institute for Immunobiology and Epigenetics, Freiburg 79108, Germany
2Department of Biological Physics, Max Planck Institute for Immunobiology and Epigenetics, Freiburg 79108, Germany
3Proteomics Facility, Max Planck Institute for Immunobiology and Epigenetics, Freiburg 79108, Germany
4Lead contact
*Corresponding author: correspondence to Ibrahim I. Cisse´
Abstract
Endogenous condensates with transient constituents are notoriously difficult to study with common biological assays like mass spectrometry and other proteomics profiling. Here, we report a method for light-induced targeting of endogenous condensates (LiTEC) in living cells. LiTEC combines the identification of molecular zip codes that target the endogenous condensates with optogenetics to enable controlled and reversible partitioning of an arbitrary cargo, such as enzymes commonly used in proteomics, into the condensate in a blue light-dependent manner. We demonstrate a proof of concept by combining LiTEC with proximity-based biotinylation (BioID) and uncover putative components of transcriptional condensates in mouse embryonic stem cells. Our approach opens the road to genome-wide functional studies of endogenous condensates.
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