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한국과학기술연구원(KIST)

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Nano Converg., Oct 15 2024, 11 (1) | https://doi.org/10.1186/s40580-024-00448-z Evaluation of size-dependent uptake, transport and cytotoxicity of polystyrene microplastic in a blood-brain barrier (BBB) model

Yeongseon Cho^1,2, Eun U Seo^1,3, Kyeong Seob Hwang¹, Hyelim Kim^1,4, Jonghoon Choi^2* and Hong Nam Kim^1,3,5,6,7*

¹Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea

²School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea

³Division of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of Korea

⁴Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea

⁵University of Science and Technology, Seoul 02792, Republic of Korea

⁶School of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea

⁷Yonsei-Korea Institute of Science and Technology Convergence Research Institute, Yonsei University, Seoul 03722, Republic of Korea

^*Correspondence: Jonghoon Choi, Hong Nam Kim

Abstract

Microplastics, particularly those in the micrometer scale, have been shown to enter the human body through ingestion, inhalation, and dermal contact. Recent research indicates that microplastics can potentially impact the central nervous system (CNS) by crossing the blood-brain barrier (BBB). However, the exact mechanisms of their transport, uptake, and subsequent toxicity at BBB remain unclear. In this study, we evaluated the size-dependent uptake and cytotoxicity of polystyrene microparticles using an engineered BBB model. Our findings demonstrate that 0.2 μm polystyrene microparticles exhibit significantly higher uptake and transendothelial transport compared to 1.0 μm polystyrene microparticles, leading to increased permeability and cellular damage. After 24 h of exposure, permeability increased by 15.6-fold for the 0.2 μm particles and 2-fold for the 1.0 μm particles compared to the control. After 72 h of exposure, permeability further increased by 27.3-fold for the 0.2 μm particles and a 4.5-fold for the 1.0 μm particles compared to the control. Notably, microplastics administration following TNF-α treatment resulted in enhanced absorption and greater BBB damage compared to non-stimulated conditions. Additionally, the size-dependent toxicity observed differently between 2D cultured cells and 3D BBB models, highlighting the importance of testing models in evaluating environmental toxicity.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-10-16)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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