구현 (University of Pennsylvania) | 한빛사논문 > 한빛사

한빛사논문

조회129

University of Pennsylvania

Adv. Healthc. Mater., Oct 14 2024, | https://doi.org/10.1002/adhm.202402306 Nanozyme-Shelled Microcapsules for Targeting Biofilm Infections in Confined Spaces

Hong Huy Tran ^1,2,3,4, Nadasinee Jaruchotiratanasakul ^1,2,5,6, Zhenting Xiang ^1,2,4, Nil Kanatha Pandey ^2,7, Min Jun Oh ^2,3,4, Yuan Liu ^1,2,8, Zhi Ren ^1,2,4, Alaa Babeer ⁹, Michael J. Zdilla ¹⁰, David P. Cormode ^7,11, Bekir Karabucak ⁵, Daeyeon Lee^1,3, Edward B. Steager ^1,2,12, Hyun Koo ^1,2,4,11

¹Center for Innovation & Precision Dentistry, School of Dental Medicine, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA, 19104, USA.

²Biofilm Research Laboratories, Levy Center for Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

³Department of Chemical and Biomolecular Engineering, School of Engineering & Applied Science, University of Pennsylvania, Philadelphia, PA, 19104, USA.

⁴Department of Orthodontics and Divisions of Pediatric Dentistry and Community Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

⁵Department of Endodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

⁶Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, 10400, Thailand.

⁷Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

⁸Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

⁹Department of Oral Biology, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.

¹⁰Department of Chemistry, Temple University, Philadelphia, PA, 19122, USA.

¹¹Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, 19104, USA.

¹²GRASP Laboratory, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Corresponding Authors: Daeyeon Lee, Edward B. Steager, Hyun Koo

H.H.T. and N.J. contributed equally to this work.

Abstract

Bacterial infections in irregular and branched confinements pose significant therapeutic challenges. Despite their high antimicrobial efficacy, enzyme-mimicking nanoparticles (nanozymes) face difficulties in achieving localized catalysis at distant infection sites within confined spaces. Incorporating nanozymes into microrobots enables the delivery of catalytic agents to hard-to-reach areas, but poor nanoparticle dispersibility and distribution during fabrication hinder their catalytic performance. To address these challenges, a nanozyme-shelled microrobotic platform is introduced using magnetic microcapsules with collective and adaptive mobility for automated navigation and localized catalysis within complex confinements. Using double emulsions produced from microfluidics as templates, iron oxide and silica nanoparticles are assembled into 100-µm microcapsules, which self-organize into multi-unit, millimeter-size assemblies under rotating magnetic fields. These microcapsules exhibit high peroxidase-like activity, efficiently catalyzing hydrogen peroxide to generate reactive oxygen species (ROS). Notably, microcapsule assemblies display remarkable collective navigation within arched and branched confinements, reaching the targeted apical regions of the tooth canal with high accuracy. Furthermore, these nanozyme-shelled microrobots perform rapid catalysis in situ and effectively kill biofilms on contact via ROS generation, enabling localized antibiofilm action. This study demonstrates a facile method of integrating nanozymes onto a versatile microrobotic platform to address current needs for targeted therapeutic catalysis in complex and confined microenvironments.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-10-22)
연구진국외 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기