한빛사논문
Elena Seibel1,2,12, Soohyun Um2,3,12, Kasun H. Bodawatta4, Anna J. Komor5, Tanya Decker1, Janis Fricke1, Robert Murphy6, Gibson Maiah7, Bulisa Iova8, Hannah Maus9, Tanja Schirmeister9, Knud Andreas Jønsson4,10, Michael Poulsen6 & Christine Beemelmanns1,2,11
1Anti-infectives from Microbiota, Helmholtz-Institut für Pharmazeutische Forschung Saarland (HIPS), Campus E8.1, 66123 Saarbrücken, Germany.
2Chemical Biology of Microbe-Host Interactions, Leibniz institute for Natural Product Research and Infection Biology – Hans-Knöll-Institute (HKI), Beutenbergstraße 11a, 07745 Jena, Germany.
3Yonsei Institute of Pharmaceutical Sciences, College of Pharmacy Yonsei University, Songdogwahak-ro 85, Incheon 21983, Republic of Korea.
4Natural History Museum of Denmark, Research and Collections University of Copenhagen, 2100 Copenhagen East, Denmark.
5Department of Biomolecular Chemistry, Leibniz institute for Natural Product Research and Infection Biology – Hans-Knöll-Institute (HKI), Beutenbergstraße 11a, 07745 Jena, Germany.
6Section for Ecology and Evolution, Department of Biology, University of Copenhagen, 2100 Copenhagen East, Denmark.
7The New Guinea Binatang Research Centre, Madang, Papua New Guinea.
8Papua New Guinea National Museum and Art Gallery, Port Moresby, Papua New Guinea.
9Institute for Pharmaceutical and Biomedical Sciences (IPBW), Johannes Gutenberg University Mainz, Staudinger Weg 5, 55128 Mainz, Germany.
10Swedish Museum of Natural History, Department of Bioinformatics and Genetics, P.O. Box 50007, SE-10405 Stockholm, Sweden.
11Saarland University, Campus, 66123 Saarbrücken, Germany.
12These authors contributed equally: Elena Seibel, Soohyun Um.
Corresponding author
Correspondence to Christine Beemelmanns.
Abstract
Uropygial gland secretions of birds consist of host and bacteria derived compounds and play a major sanitary and feather-protective role. Here we report on our microbiome studies of the New Guinean toxic bird Pachycephala schlegelii and the isolation of a member of the Amycolatopsis genus from the uropygial gland secretions. Bioactivity studies in combination with co-cultures, MALDI imaging and HR-MS/MS-based network analyses unveil the basis of its activity against keratinolytic bacteria and fungal skin pathogens. We trace the protective antimicrobial activity of Amycolatopsis sp. PS_44_ISF1 to the production of rifamycin congeners, ciromicin A and of two yet unreported compound families. We perform NMR and HR-MS/MS studies to determine the relative structures of six members belonging to a yet unreported lipopeptide family of pachycephalamides and of one representative of the demiguisins, a new hexapeptide family. We then use a combination of phylogenomic, transcriptomic and knock-out studies to identify the underlying biosynthetic gene clusters responsible for the production of pachycephalamides and demiguisins. Our metabolomics data allow us to map molecular ion features of the identified metabolites in extracts of P. schlegelii feathers, verifying their presence in the ecological setting where they exert their presumed active role for hosts. Our study shows that members of the Actinomycetota may play a role in avian feather protection.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기