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New Phytol., Oct 10 2024, | https://doi.org/10.1111/nph.20169
MpPUB9, a U-box E3 ubiquitin ligase, acts as a positive regulator by promoting the turnover of MpEXO70.1 under high salinity in Marchantia polymorpha
Cheol Jin Lim1,2*, Hyeon Ji Seo1,2*, Haijing Yin1,2, Na Hyun Cho1,2, Hee Woong Yang1,2, Tae Hyeon Park1,2, Yun Ju Kim1,2, Woo Taek Kim1,2 and Dong Hye Seo1,2
1Department of Systems Biology and Division of Life Science, Yonsei University, Seoul, 03722, Korea;
2Institute of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Korea
*These authors contributed equally to this work.
Authors for correspondence: Dong Hye Seo, Woo Taek Kim
Abstract
Marchantia polymorpha, occupying a basal position in the monophyletic assemblage of land plants, displays a notable expansion of plant U-box (PUB) proteins compared with those in animals. We elucidated the roles of MpPUB9 in regulating salt stress tolerance in M. polymorpha. MpPUB9 expression was rapidly induced by high salinity and dehydration. MpPUB9 possessed an intact U-box domain in the N-terminus. MpPUB9-Citrine localized to punctate structures and was peripherally associated with microsomal membranes. Phenotypic analyses demonstrate that the hyponastic and epinastic thallus growth phenotypes, which were induced by the overexpression and suppression of MpPUB9, may provoke salt stress-resistant and -susceptible phenotypes, respectively. MpPUB9 was also found to directly interact with the exocyst protein MpEXO70.1, leading to its ubiquitination. Under high-salinity conditions, though the stability of MpPUB9 was dramatically increased, MpEXO70.1 showed slightly faster turnover rates. Transcriptome analyses showed that salt treatment and the overexpression of MpPUB9 co-upregulated the genes related to the modulation of H2O2 and cell wall organization. Overall, our results suggest that MpPUB9 plays a crucial role in the positive regulation of salt stress tolerance, resulting from its interaction with MpEXO70.1 and modulating turnover of the protein under high-salt conditions via the coordination of UPS with autophagy.
논문정보
- Research article
- 2024년 10월 (BRIC 등록일 2024-10-17)
- 국내 연구진
- 생명과학 > 식물학
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