목혜정 (건국대학교) | 한빛사논문 > 한빛사

한빛사논문

조회293

건국대학교

CV File 111 kb

J. Control. Release, Available online 9 October 2024 | https://doi.org/10.1016/j.jconrel.2024.09.053 Milk exosome-infused fibrous matrix for treatment of acute wound

Hoai-Thuong Duc Bui ^a,1, Gayeon You ^b,1, Miso Lee ^a,1, Wei Mao ^a, Chaewon So ^b, Chorok Byeon ^c, Seonki Hong ^c, Hyejung Mok ^b, Hyuk Sang Yoo ^a,d,e,f

^aDepartment of Medical Biomaterials Engineering, Kangwon National University, Chuncheon 24341, Republic of Korea

^bDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea

^cDepartment of Physics and Chemistry, DGIST, Daegu, 42988, Republic of Korea

^dInstitute for Molecular Science and Fusion Technology, Kangwon National University, Chuncheon 24341, Republic of Korea

^eInstitute of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea

^fKangwon Radiation Convergence Research Center, Kangwon National University, Chuncheon 24341, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: Hyejung Mok, Hyuk Sang Yoo

Abstract

To provide an advanced therapy for wound recovery, in this study, pasteurized bovine milk-derived exosomes (mEXO) are immobilized onto a polydopamine (PDA)-coated hyaluronic acid (HA)-based electrospun nanofibrous matrix (mEXO@PMAT) via a simple dip-coating method to formulate an mEXO-immobilized mesh as a wound-healing biomaterial. Purified mEXOs (~82 nm) contain various anti-inflammatory, cell proliferation, and collagen synthesis-related microRNAs (miRNAs), including let-7b, miR-184, and miR-181a, which elicit elevated mRNA expression of keratin5, keratin14, and collagen1 in human keratinocytes (HaCaT) and fibroblasts (HDF). The mEXOs immobilized onto the PDA-coated meshes are gradually released from the meshes over 14 days without burst-out effect. After treatment with HaCaT and HDF, the degree of in vitro cell migration increases significantly in the mEXO@PMAT-treated HaCaT and HDF cells compared to the unmodified or PDA-coated meshes-treated cells. Additionally, the mEXO@PMAT provides significantly faster wound closure in vivo without notable toxicity. Thus, the sustained liberation of bioactive mEXO from the meshes can effectively enhance cell proliferation in vitro and accelerate wound closure in vivo, which could be harnessed mEXO@PMAT as a promising wound-healing biomaterial.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-10-11)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

댓글 작성 로그인

CV 열람하기

연락처 보기