이주아 (Northwestern University, 현 Boston University) | 한빛사논문 > 한빛사

한빛사논문

조회1,772

Northwestern University, 현 Boston University

Nat. Commun., 07 October 2024 | https://doi.org/10.1038/s41467-024-53032-8 Deciphering mouse brain spatial diversity via glyco-lipidomic mapping

Jua Lee ¹, Dongtan Yin^2,3, Jaekyung Yun^2,3, Minsoo Kim ⁴, Seong-Wook Kim ⁴, Heeyoun Hwang ⁵, Ji Eun Park^2,3, Boyoung Lee ⁴, C. Justin Lee ⁴, Hee-Sup Shin ⁴& Hyun Joo An ^2,3,*

¹Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
²Graduate School of Analytical Science & Technology, Chungnam National University, 34134 Daejeon, South Korea.
³Asia-Pacific Glycomics Reference Site, 34134 Daejeon, South Korea.
⁴Center for Cognition and Sociality, Institute for Basic Science, 34051 Daejeon, South Korea.
⁵Research Center for Bioconvergence Analysis, Korea Basic Science Institute, 28119 Cheongju, South Korea.

^*Corresponding author: correspondence to Hyun Joo An

Abstract

Gangliosides in the brain play a crucial role in modulating the integrity of vertebrate central nervous system in a region-specific manner. However, to date, a comprehensive structural elucidation of complex intact ganglioside isomers has not been achieved, resulting in the elusiveness into related molecular mechanism. Here, we present a glycolipidomic approach for isomer-specific and brain region-specific profiling of the mouse brain. Considerable region-specificity and commonality in specific group of regions are highlighted. Notably, we observe a similarity in the abundance of major isomers, GD1a and GD1b, within certain regions, which provides significant biological implications with interpretation through the lens of a theoretical retrosynthetic state-transition network. Furthermore, A glycocentric-omics approaches using gangliosides and N-glycans reveal a remarkable convergence in spatial dynamics, providing valuable insight into molecular interaction network. Collectively, this study uncovers the spatial dynamics of intact glyco-conjugates in the brain, which are relevant to regional function and accelerates the discovery of potential therapeutic targets for brain diseases.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-10-08)
연구진국내(교신)+국외 연구진
분야 생명과학 > 생화학

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