정원 (Harvard University) | 한빛사논문 > 한빛사

한빛사논문

조회196

Harvard University

CV File 456 kb

Proc. Natl. Acad. Sci. USA, 2024 Oct 8;121(41):e2407647121 | https://doi.org/10.1073/pnas.2407647121 Memory effects of transcription regulator−DNA interactions in bacteria

Won Jung ^{1 2 *}, Tai-Yen Chen^{1 3}, Ace George Santiago^{1 4}, Peng Chen^{1 *}

¹Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853.

²Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138.

³Department of Chemistry, University of Houston, Houston, TX 77204.

⁴10x Genomics, Pleasanton, CA 94588.

^*Corresponding authors: correspondence to Won Jung or Peng Chen

Abstract

Memory effect refers to the phenomenon where past events influence a system's current and future states or behaviors. In biology, memory effects often arise from intra- or intermolecular interactions, leading to temporally correlated behaviors. Single-molecule studies have shown that enzymes and DNA-binding proteins can exhibit time-correlated behaviors of their activity. While memory effects are well documented and studied in vitro, no such examples exist in cells to our knowledge. Combining single-molecule tracking (SMT) and single-cell protein quantitation, we find in living Escherichia coli cells distinct temporal correlations in the binding/unbinding events on DNA by MerR- and Fur-family metalloregulators, manifesting as memory effects with timescales of ~1 s. These memory effects persist irrespective of the type of the metalloregulators or their metallation states. Moreover, these temporal correlations of metalloregulator-DNA interactions are associated with spatial confinements of the metalloregulators near their DNA binding sites, suggesting microdomains of ~100 nm in size that possibly result from the spatial organizations of the bacterial chromosome without the involvement of membranes. These microdomains likely facilitate repeated binding events, enhancing regulator-DNA contact frequency and potentially gene regulation efficiency. These findings provide unique insights into the spatiotemporal dynamics of protein-DNA interactions in bacterial cells, introducing the concept of microdomains as a crucial player in memory effect-driven gene regulation.

논문정보

형식Research article
게재일2024년 10월 (BRIC 등록일 2024-10-31)
연구진국외 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기