한빛사논문
- 조회187
Yoon-Tae Kang 1,8, Ji-Young Kim 1,2,4,8, Emine Sumeyra Turali-Emre 1, Abha Kumari 1, Hee-Jeong Jang 1, Minjeong Cha 1, Colin Palacios-Rolston 1, Chitra Subramanian 1, Emma Purcell 1, Sarah Owen 1, Chung-Man Lim 1, Rishindra Reddy 5, Shruthi Jolly 6, Nithya Ramnat 7, Sunitha Nagrath 1,3,9, Nicholas A. Kotov 1,2
1Department of Chemical Engineering and Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
2Center for Complex Particle Systems (COMPASS), University of Michigan, Ann Arbor, MI 48109, USA
3Roger Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA
4Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
5Michigan Medicine Thoracic Surgery Clinic, Ann Arbor, MI 48109, USA
6Radiation of Oncology, University of Hospital, University of Michigan, Ann Arbor, MI 48109, USA
7Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA
8These authors contributed equally
9Lead contact
Corresponding authors: Yoon-Tae Kang, Ji-Young Kim, Sunitha Nagrath, Nicholas A. Kotov
Abstract
Cancer-cell-secreted small extracellular vesicles, known as exosomes, represent a rapidly emerging family of cancer biomarkers. However, the current protocols for exosome analysis require complex equipment and lengthy procedures, which prevents their broad utilization for cancer diagnosis. We have engineered plasmonic gold nanoparticles combining molecular and nanoscale chirality, and have demonstrated that such nanoparticles in self-assembled films in a microfluidic device can isolate and analyze exosomes directly from blood plasma due to marker-specific chiroptical responses and volumetric electromagnetic resonance. Cancer exosomes can be distinguished from those from healthy donors by their giant polarization rotation signatures, and the observed dependence of plasmonic resonances on mutations of epidermal growth factor receptor suggests the possibility of in-line mutation/deletion analysis of protein cargo based on molecular chirality. The present microfluidic chips eliminate ultracentrifugation and improve the sensitivity and detection speed by at least 14 times and 10 times, respectively, enabling the rapid liquid biopsy of cancer.
논문정보
- Research article
- 2024년 09월 (BRIC 등록일 2024-10-14)
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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