한빛사논문
- 조회255
Young Geun Mok 1,3, Sunghyun Hong 1,3, Da In Seo 1, Seunghee Choi 1 , Hee Kyoung Kim 1, Da Mon Jin 1, JungEun Joanna Lee 1 & Jin-Soo Kim 1,2,*
1GreenGene, Inc., Seoul, Republic of Korea.
2NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI) and Department of Biochemistry, National University of Singapore, Singapore, Singapore.
3These authors contributed equally: Young Geun Mok, Sunghyun Hong.
*Corresponding author: correspondence to Jin-Soo Kim
Abstract
CRISPR-free, protein-only cytosine base editors (CBEs) or adenine base editors, composed of DNA-binding proteins such as zinc finger proteins or transcription activator-like effectors (TALEs) and nucleobase cytosine or adenine deaminases, respectively, enable organellar DNA editing in cultured cells, animals and plants. TALE-linked double-stranded DNA deaminase toxin A (DddAtox)-derived CBEs (DdCBEs) and TALE-linked adenine deaminases (TALEDs) install C-to-T and A-to-G single-nucleotide conversions, respectively, in mitochondria and chloroplasts. Interestingly, whereas TALEDs exclusively induce A-to-G conversions without C-to-T conversions in mammalian mitochondrial DNA, they often install unwanted C-to-T edits in addition to intended A-to-G edits in plastid DNA. Here we show that uracil DNA glycosylase (UDG)-fused TALEDs (UDG-TALEDs) minimize C-to-T conversions without reducing the A-to-G editing efficiency and install a mutation in the chloroplast psbA gene that encodes a single-amino-acid substitution (S264G), which confers herbicide resistance in the resulting plants.
논문정보
- Letter
- 2024년 09월 (BRIC 등록일 2024-10-17)
- 국내(교신)+국외 연구진
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
