이재성 (성균관대학교)

한빛사논문

조회82

성균관대학교

CV File 747 kb

Environ. Sci. Technol. , Sep 11 2024, | https://doi.org/10.1021/acs.est.4c05105 Molecular Events in Response to Triclosan-Induced Oxidative Stress in CRISPR/Cas9-Mediated p53-Targeted Mutants in Daphnia magna

Jin-Sol Lee ¹, Ju Ri Kim², Eunjin Byeon¹, Duck-Hyun Kim ¹, Hyung Sik Kim ², Jae-Seong Lee ¹

¹Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.

²School of Pharmacy, Sungkyunkwan University, Suwon 16419, South Korea.

Corresponding Authors : Hyung Sik Kim, Jae-Seong Lee

Abstract

Triclosan (TCS), a widely used antimicrobial agent, has been implicated in the oxidative stress induction and disruption of cellular processes in aquatic organisms. As TCS is ubiquitous in the aquatic environment, many previous studies have documented the effects of exposure to TCS on aquatic organisms. Nevertheless, most of the research has concentrated on the molecular and physiological responses of TCS, but there are still limited studies on the function of specific genes and the consequences of their absence. In this study, we focused on p53, a gene that is crucial for molecular responses such as autophagy and apoptosis as a result of TCS exposure. In order to ascertain the role and impact of the p53 gene in TCS-induced molecular responses, we examined the molecular responses to TCS-induced oxidative stress in wild-type (WT) and CRISPR/Cas9-mediated p53 mutant (MT) water fleas. The result has been accomplished by examining changes in molecular mechanisms, including in vivo end points, enzyme activities, adenosine triphosphate release rate, and apoptosis, to determine the role and impact of the p53 gene on TCS-induced molecular responses. The results indicated that the sensitivity of MT water fleas to TCS was greater than that of WT water fleas; however, the difference in sensitivity was significant at short exposures within 48 h and decreased toward 48 h. Accordingly, when we confirmed the oxidative stress after 24 h of exposure, the oxidative stress to TCS exposure was stronger in the MT group, with an imbalance of redox. To identify the mechanisms of tolerance to TCS in WT and MT Daphnia magna, we checked mitochondrial and ER-stress-related biomarkers and found an increase in apoptosis and greater sensitivity to TCS exposure in the MT group than in the WT. Our results suggest that the absence of p53 caused alterations in molecular processes in response to TCS exposure, resulting in increased sensitivity to TCS, and that p53 plays a critical role in response to TCS exposure.

논문정보

형식Research article
게재일2024년 09월 (BRIC 등록일 2024-09-12)
연구진국내 연구진
분야 생명과학 > 분류/생태/환경생물학

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