박재형 (성균관대학교)

한빛사논문

조회35

성균관대학교

CV File 345 kb

J. Control. Release, Sep 05 2024, 375 S0168-3659(24)00600-X | https://doi.org/10.1016/j.jconrel.2024.08.050 Chondroitin sulfate-based microneedles for transdermal delivery of stem cell-derived extracellular vesicles to treat rheumatoid arthritis

Van Dat Bui ^a,1, Jueun Jeon ^a,1, Van Hieu Duong ^a, Sol Shin ^a, Jungmi Lee ^a, Farrokhroo Ghahari^a, Chan Ho Kim ^a, Yu Jin Jo ^a, Won-Kyo Jung ^b,c, Wooram Um^d, Jae Hyung Park^a,e

^aSchool of Chemical Engineering, College of Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea

^bMarine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea

^cMajor of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea

^dDepartment of Biotechnology, College of Fisheries Science, Pukyong National University, Busan 48513, Republic of Korea

^eBiomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea

¹Van Dat Bui and Jueun Jeon contributed equally to this work.

Corresponding authors: Wooram Um, Jae Hyung Park

Abstract

For the non-invasive treatment of rheumatoid arthritis (RA), a chondroitin sulfate C (CSC)-based dissolving microneedles (cMN) was prepared to deliver human adipose stem cell-derived extracellular vesicles (hASC-EV) into inflamed joints. Owing to their anti-inflammatory function, the hASC-EV-bearing cMN (EV@cMN) significantly suppressed activated fibroblast-like synoviocytes (aFLS) and M1 macrophages (M1), which are responsible for the progression of RA. In addition, EV@cMN facilitated the chondrogenic differentiation of bone marrow-derived stem cells. In mice with collagen-induced arthritis, EV@cMN efficiently delivered both hASC-EV and CSC to inflamed joints. Interestingly, pro-inflammatory cytokines in the inflamed joints were remarkably downregulated by the synergistic effect of CSC and hASC-EV. Consequently, as judged from the overall clinical score and joint swelling, EV@cMN showed an outstanding therapeutic effect, even comparable to the wild-type mice, without significant adverse effects. Overall, EV@cMN might have therapeutic potential for RA by efficiently delivering CSC and hASC-EV into the inflamed joints in a non-invasive manner.

논문정보

형식Research article
게재일2024년 09월 (BRIC 등록일 2024-09-20)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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