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Ngan Thi NguyenNgan Thi Nguyen
경상국립대학교
Nat. Plants, Sep 06 2024, | https://doi.org/10.1038/s41477-024-01786-w
Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis
Tien Van Vu1,5, Ngan Thi Nguyen1,5, Jihae Kim1, Young Jong Song1, Thu Hoai Nguyen1,2 & Jae-Yean Kim1,3,4
1Division of Applied Life Science (BK21 Four Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Republic of Korea.
2Forest Protection Research Centre, Vietnamese Academy of Forest Sciences, Hanoi, Vietnam.
3Division of Life Science, Gyeongsang National University, Jinju, Republic of Korea.
4Nulla Bio R&D Center, Nulla Bio Inc, Jinju, Republic of Korea.
5These authors contributed equally: Tien Van Vu, Ngan Thi Nguyen.
Corresponding authors
Correspondence to Tien Van Vu or Jae-Yean Kim.
Abstract
Prime editing (PE) enables almost all types of precise genome editing in animals and plants. It has been successfully adapted to edit several plants with variable efficiency and versatility. However, this technique is inefficient for dicots for unknown reasons. Here, using new combinations of PE components, including an RNA chaperone and altered engineered prime editing guide RNAs driven by a PolII-PolIII composite promoter and a viral replicon system, we obtained up to 9.7% of the desired PE efficiency at the callus stage as assessed by targeted deep sequencing. Subsequently, we identified that up to 38.2% of transformants contained desired PE alleles in tomatoes and Arabidopsis, marking successful heritable PE transmission. Our PE tools also showed high accuracy, specificity and multiplexing capability, which unlocked the potential for practical PE applications in dicots, paving the way for transformative advancements in plant sciences.
논문정보
- Research article
- 2024년 09월 (BRIC 등록일 2024-09-09)
- 국내(교신)+국외 연구진
- 생명과학 > 식물학
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