김나연 (연세대학교)

한빛사논문

조회94

김나연Nayeon Kim

연세대학교

Gut Microbes, Published online: 04 Sep 2024 | https://doi.org/10.1080/19490976.2024.2393791 MRGM: an enhanced catalog of mouse gut microbial genomes substantially broadening taxonomic and functional landscapes

Nayeon Kim ^a, Chan Yeong Kim^a, Junyeong Ma^a, Sunmo Yang^a, Dong Jin Park^b, Sang-Jun Ha^b, Peter Belenky ^c,and Insuk Lee^a,d

^aDepartment of Biotechnology, College of Life Science & Biotechnology, Yonsei University, Seoul, Republic of Korea;

^bDepartment ofBiochemistry, College of Life Science & Biotechnology, Yonsei University, Seoul, Republic of Korea;

^cDepartment of Molecular Microbiologyand Immunology, Brown University, Providence, RI, USA;

^dPOSTECH Biotech Center, Pohang University of Science and Technology (POSTECH),Pohang, Republic of Korea

CONTACT: Insuk Lee, Peter Belenky

Abstract

Mouse gut microbiome research is pivotal for understanding the human gut microbiome, providing insights into disease modeling, host-microbe interactions, and the dietary influence on the gut microbiome. To enhance the translational value of mouse gut microbiome studies, we need detailed and high-quality catalogs of mouse gut microbial genomes. We introduce the Mouse Reference Gut Microbiome (MRGM), a comprehensive catalog with 42,245 non-redundant mouse gut bacterial genomes across 1,524 species. MRGM marks a 40% increase in the known taxonomic diversity of mouse gut microbes, capturing previously underrepresented lineages through refined genome quality assessment techniques. MRGM not only broadens the taxonomic landscape but also enriches the functional landscape of the mouse gut microbiome. Using deep learning, we have elevated the Gene Ontology annotation rate for mouse gut microbial proteins from 3.2% with orthology to 60%, marking an over 18-fold increase. MRGM supports both DNA- and marker-based taxonomic profiling by providing custom databases, surpassing previous catalogs in performance. Finally, taxonomic and functional comparisons between human and mouse gut microbiota reveal diet-driven divergences in their taxonomic composition and functional enrichment. Overall, our study highlights the value of high-quality microbial genome catalogs in advancing our understanding of the co-evolution between gut microbes and their host.

논문정보

형식Research article
게재일2024년 09월 (BRIC 등록일 2024-09-05)
연구진국내(교신)+국외 연구진
분야 생명과학 > 유전학

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