정상민 (서울대학교)

한빛사논문

조회104

정상민Sangmin Jung

서울대학교

ACS Nano, Aug 29 2024, | https://doi.org/10.1021/acsnano.4c05537 Integrating Vascular Phenotypic and Proteomic Analysis in an Open Microfluidic Platform

Sangmin Jung ¹, Sunghun Cheong ², Yoonho Lee ², Jungseub Lee ¹, Jihye Lee ³, Min-Seok Kwon ^3,4, Young Sun Oh ^1,3, Taewan Kim⁵, Sungjae Ha⁶, Sung Jae Kim^5,7,8, Dong Hyun Jo ⁹, Jihoon Ko ¹⁰, Noo Li Jeon^1,2,11,12

¹Department of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea.

²Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.

³Target Link Therapeutics, Inc., Seoul 04545, Republic of Korea.

⁴Department of Public Health Science, Graduate School of Public Health, Seoul National University, Seoul 08826, Republic of Korea.

⁵Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of Korea.

⁶ProvaLabs, Inc., Seoul 08826, Republic of Korea.

⁷SOFT Foundry, Seoul National University, Seoul 08826, Republic of Korea.

⁸Inter-university Semiconductor Research Center, Seoul National University, Seoul 08826, Republic of Korea.

⁹Department of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.

¹⁰Department of BioNano Technology, Gachon University, Seongnam-si, Gyeonggi-do 13120, Republic of Korea.

¹¹Institute of Advanced Machines and Design, Seoul National University, Seoul 08826, Republic of Korea.

¹²Qureator, Inc., San Diego, California 92121, United States.

Corresponding Authors: Jihoon Ko, Noo Li Jeon

Abstract

This research introduces a vascular phenotypic and proteomic analysis (VPT) platform designed to perform high-throughput experiments on vascular development. The VPT platform utilizes an open-channel configuration that facilitates angiogenesis by precise alignment of endothelial cells, allowing for a 3D morphological examination and protein analysis. We study the effects of antiangiogenic agents─bevacizumab, ramucirumab, cabozantinib, regorafenib, wortmannin, chloroquine, and paclitaxel─on cytoskeletal integrity and angiogenic sprouting, observing an approximately 50% reduction in sprouting at higher drug concentrations. Precise LC-MS/MS analyses reveal global protein expression changes in response to four of these drugs, providing insights into the signaling pathways related to the cell cycle, cytoskeleton, cellular senescence, and angiogenesis. Our findings emphasize the intricate relationship between cytoskeletal alterations and angiogenic responses, underlining the significance of integrating morphological and proteomic data for a comprehensive understanding of angiogenesis. The VPT platform not only advances our understanding of drug impacts on vascular biology but also offers a versatile tool for analyzing proteome and morphological features across various models beyond blood vessels.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-09-02)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오·의료융합 기타

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