한빛사논문
- 조회85
Jong-Hwan Lee1,2, Jihwan Song3, SoonGweon Hong1,4, Yun Kim3, Minsun Song1,4, Byungrae Cho1, Tiffany Wu5, Lee W. Riley5, Ulf Landegren6, and Luke P. Lee1,4,7,8*
1Department of Bioengineering, University of California Berkeley, Berkeley, CA, 94720 USA
2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, 34114 South Korea
3Department of Mechanical Engineering, Hanbat National University, Daejeon, 34158 South Korea
4Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115 USA
5Division of Infectious Disease and Vaccinology, School of Public Health, University of California, Berkeley, CA, 94720 USA
6Departments of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SE-751 08 Sweden
7Department of Electrical Engineering and Computer Science, University of California Berkeley, Berkeley, CA, 94720 USA
8Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, 16419 South Korea
J.H.L. and J.S. contributed equally to this work.
Corresponding Author : Luke P. Lee
Abstract
The emergence of antibiotic resistance has become a global health crisis, and everyone must arm themselves with wisdom to effectively combat the “silent tsunami” of infections that are no longer treatable with antibiotics. However, the overuse or inappropriate use of unnecessary antibiotics is still routine for administering them due to the unavailability of rapid, precise, and point-of-care assays. Here, a rapid antimicrobial-resistance point-of-care identification device (RAPIDx) is reported for the accurate and simultaneous identification of bacterial species (genotype) and target enzyme activity (phenotype). First, a contamination-free active target enzyme is extracted via the photothermal lysis of preconcentrated bacteria cells on a nanoplasmonic functional layer on-chip. Second, the rapid, precise identification of pathogens is achieved by the photonic rolling circle amplification of DNA on a chip. Third, the simultaneous identification of bacterial species (genotype) and target enzyme activity (phenotype) is demonstrated within a sample-to-answer 45 min operation via the RAPIDx. It is believed that the RAPIDx will be a valuable method for solving the bottleneck of employing on-chip nanotechnology for antibiotic-resistant bioassay and other infectious diseases.
논문정보
- Research article
- 2024년 08월 (BRIC 등록일 2024-08-30)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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