김선필 (기초과학연구원)

한빛사논문

조회153

기초과학연구원

Redox Biol., Aug 20 2024, 76 S2213-2317(24)00298-2 | https://doi.org/10.1016/j.redox.2024.103320 Dopamine-modified hyaluronic acid (DA-HA) as a novel dopamine-mimetics with minimal autoxidation and cytotoxicity

Sunpil Kim ^a,1, Ye-Ji Kim^b,c,1, Kyoung Hwan Park ^d,e, Kang Moo Huh^d, Sun-Woong Kang ^b,e, C. Justin Lee^a, Dong Ho Woo ^b,c

^aCenter for Cognition and Sociality, Life Science Cluster, Institute for Basic Science (IBS), Daejeon, 34126, South Korea

^bHuman and Environmental Toxicology, University of Science and Technology (UST), Daejeon, 34114, South Korea

^cDepartment of Advanced Toxicology Research, Korea Institute of Toxicology (KIT), KRICT, Daejeon, 34114, South Korea

^dDepartment of Polymer Science and Engineering, Chungnam National University (CNU), Daejeon, 34134, South Korea

^eResearch Group for Biomimetic Advanced Technology, Korea Institute of Toxicology (KIT), KRICT, Daejeon, 34114, South Korea

¹These authors contributed equally to this work.

Corresponding authors: C. Justin Lee, Dong Ho Woo

Abstract

Dopamine-modified hyaluronic acid (DA-HA) has been initially developed as an efficient coating and adhesion material for industrial uses. However, the biological activity and safety of DA-HA in the brain have not been explored yet. Here, we report a series of evidence that DA-HA exhibits similar functionality as dopamine (DA), but with much lower toxicity arising from autoxidation. DA-HA shows very little autoxidation even after 48-h incubation. This is profoundly different from DA and its derivatives including l-DOPA, which all induce severe neuronal death after pre-autoxidation, indicating that autoxidation is the cause of neuronal death. Furthermore, in vivo injection of DA-HA induces significantly lower toxicity compared to 6-OHDA, a well-known oxidized and toxic form of DA, and alleviates the apomorphine-induced rotational behavior in the 6-OHDA animal model of Parkinson's disease. Our study proposes that DA-HA with DA-like functionalities and minimal toxicity has a great potential to treat DA-related disease.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-28)
연구진국내 연구진
분야 생명과학 > 신경생물학

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