남영주 (한양대학교)

한빛사논문

조회70

남영주Youngju Nam

한양대학교

Biosens. Bioelectron., Aug 18 2024, 264 S0956-5663(24)00692-4 | https://doi.org/10.1016/j.bios.2024.116686 Bioelectronic sensing platform emulating the human endocannabinoid system for assessing and modulating of cannabinoid activity

Hyunju Cho ^a,1, Da Eun Oh ^a,1, Youngju Nam ^b,1, Seung Hwan Lee ^b, Tae Hyun Kim ^a

^aDepartment of Chemistry, Soonchunhyang University, Asan, 31538, Republic of Korea

^bDepartment of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan, 15588, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: Seung Hwan Lee, Tae Hyun Kim

Abstract

Cannabinoids are involved in physiological and neuromodulatory processes through their interactions with the human cannabinoid receptor-based endocannabinoid system. Their association with neurodegenerative diseases and brain reward pathways underscores the importance of evaluating and modulating cannabinoid activity for both understanding physiological mechanisms and developing therapeutic drugs. The use of agonists and antagonists could be strategic approaches for modulation. In this study, we introduce a bioelectronic sensor designed to monitor cannabinoid binding to receptors and assess their agonistic and antagonistic properties. We produced human cannabinoid receptor 1 (hCB1R) via an Escherichia coli expression system and incorporated it into nanodiscs (NDs). These hCB1R-NDs were then immobilized on a single-walled carbon nanotube field-effect transistor (swCNT-FET) to construct a bioelectronic sensing platform. This novel system can sensitively detect the cannabinoid ligand anandamide (AEA) at concentrations as low as 1 fM, demonstrating high selectivity and real-time response. It also successfully identified the hCB1R agonist Δ⁹-tetrahydrocannabinol and observed that the hCB1R antagonist rimonabant diminished the sensor signal upon AEA binding, indicating the antagonism-based modulation of ligand interaction. Consequently, our bioelectronic sensing platform holds potential for ligand detection and analysis of agonism and antagonism.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-28)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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