이은희 (부산대학교)

한빛사논문

조회56

부산대학교

J. Hazard. Mater., Aug 09 2024, 478 S0304-3894(24)02042-9 | https://doi.org/10.1016/j.jhazmat.2024.135463 Interference-resistant gold nanoparticle assay for detecting Enterococcus in fresh and marine waters

Yunsoo Chang ^a, Ji-Hyeon Sung ^b, Seung-Woo Lee ^b,c,d, Eun-Hee Lee^a,e

^aDepartment of Microbiology, Pusan National University, 2 Busandaehak-ro 63 Beon-gil, Geumjeong-gu, Busan, Republic of Korea

^bDepartment of Fine Chemistry, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, Republic of Korea

^cDepartment of Nano Bio Engineering, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, Republic of Korea

^dCenter for Functional Biomaterials, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul, Republic of Korea

^eInstitute for Future Earth, Pusan National University, 2 Busandaehak-ro 63 Beon-gil, Geumjeong-gu, Busan, Republic of Korea

Corresponding author : Eun-Hee Lee

Abstract

Enterococci are common indicators of fecal contamination and are used to assess the quality of fresh and marine water, sand, soil, and sediment. However, samples collected from these environments contain various cells and other factors that can interfere with the assays used to detect enterococci. We developed a novel assay for the sensitive and specific detection of enterococci that is resistant to interference from other cells and environmental factors. Our interference-resistant assay used 30-nm gold nanoparticles (AuNPs), streptavidin, and a biotinylated Enterococcus antibody. Enterococci inhibited the interaction between streptavidin and biotin and led to the disaggregation of AuNPs. The absence of enterococci led to the aggregation of AuNPs, and this difference was easily detected by spectrophotometry. This interference-resistant AuNP assay was able to detect whole cells of Enterococcus in the range of 10 to 10⁷ CFU/mL within 3 h, had high specificity for enterococci, and was unaffected by the presence of other intestinal bacteria, such as Escherichia coli. Our examination of fresh and marine water samples demonstrated no interference from other cells or environmental factors. The interference-resistant AuNP assay described here has the potential to be used as a rapid, simple, and effective method for monitoring enterococci in diverse environmental samples.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-09-03)
연구진국내 연구진
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