한빛사
>
한빛사논문
한빛사논문
- 조회415
Biosens. Bioelectron., Aug 17 2024, 264 S0956-5663(24)00685-7 | https://doi.org/10.1016/j.bios.2024.116679
Bioelectronics for bitterness-based phytocompound detection using human bitter taste receptor nanodiscs
Kyung Ho Kim a, Sung Eun Seo a, Seung Hwan Lee b, Oh Seok Kwon a,c,d
aSKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, 16419, Republic of Korea
bDepartment of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, 15588, Ansan, Republic of Korea
cDepartment of Nano Science and Technology, Sungkyunkwan University, Suwon, 16419, Republic of Korea
dDepartment of Nano Engineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea
Corresponding authors: Seung Hwan Lee, Oh Seok Kwon
Abstract
Various organisms produce several products to defend themselves from the environment and enemies. These natural products have pharmacological and biological activities and are used for therapeutic purposes, retaining bitter taste because of chemical defense mechanisms. Cnicin is a plant-derived bitter sesquiterpene lactone with pharmacological characteristics such as anti-bacterial, anti-myeloma, anti-cancer, anti-tumor, anti-oxidant, anti-inflammatory, allelopathic, and cytotoxic properties. Although many studies have focused on cnicin detection, they have limitations and novel cnicin-detecting strategies are required. In this study, we developed the bioelectronics for screening cnicin using its distinct taste. hTAS2R46 was produced using an Escherichia coli expression system and reconstituted into nanodiscs (NDs). The binding sites and energy between hTAS2R46 and cnicin were investigated using biosimulations. hTAS2R46-NDs were combined with a side-gated graphene micropatterned field-effect transistor (SGMFET) to construct hTAS2R46-NDs bioelectronics. The construction was examined by chemical and electrical characterization. The developed system exhibited unprecedented performance, 10 fM limit of detection, rapid response time (within 10 s), 0.1354 pM-1 equilibrium constant, and high selectivity. Furthermore, the system was stable as the sensing performance was maintained for 15 days. Therefore, the hTAS2R46-NDs bioelectronics can be utilized to screen cnicin from natural products and applied in the food and drug industries.
논문정보
- Research article
- 2024년 08월 (BRIC 등록일 2024-08-29)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기
