한빛사논문
- 조회1,044
Ivo Severins 1 2, Carolien Bastiaanssen 1, Sung Hyun Kim 1 3, Roy B Simons 1, John van Noort 2 *, Chirlmin Joo 1 3 *
1Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands.
2Biological and Soft Matter Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, Netherlands.
3Department of Physics, Ewha Womans University, Seoul 03760, Republic of Korea.
*Corresponding authors: correspondence to John van Noort or Chirlmin Joo
Abstract
At the core of molecular biology lies the intricate interplay between sequence, structure, and function. Single-molecule techniques provide in-depth dynamic insights into structure and function, but laborious assays impede functional screening of large sequence libraries. We introduce high-throughput Single-molecule Parallel Analysis for Rapid eXploration of Sequence space (SPARXS), integrating single-molecule fluorescence with next-generation sequencing. We applied SPARXS to study the sequence-dependent kinetics of the Holliday junction, a critical intermediate in homologous recombination. By examining the dynamics of millions of Holliday junctions, covering thousands of distinct sequences, we demonstrated the ability of SPARXS to uncover sequence patterns, evaluate sequence motifs, and construct thermodynamic models. SPARXS emerges as a versatile tool for untangling the mechanisms that underlie sequence-specific processes at the molecular scale.
논문정보
- Research article
- 2024년 08월 (BRIC 등록일 2024-08-23)
- 국내(교신)+국외 연구진
- 생명과학 > 구조생물 및 생물물리학
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