한화승 (강릉원주대학교, 한국과학기술연구원)

한빛사논문

조회82

강릉원주대학교, 한국과학기술연구원

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J. Extracell. Vesicles, Aug 2024, 13 (8) | https://doi.org/10.1002/jev2.12487 Roseburia intestinalis-derived extracellular vesicles ameliorate colitis by modulating intestinal barrier, microbiome, and inflammatory responses

Hwa Seung Han ^1,2, Soonjae Hwang², Seung Young Choi ³, Emmanuel Hitayezu ², Mabwi A Humphrey ², Altai Enkhbayar ², Dae-Geun Song ², Myungsuk Kim ², Jong-Sung Park ³, Young-Tae Park ⁴, Jin-Soo Park², Kwang Hyun Cha ², Ki Young Choi ^1,2,3

¹Department of Marine Bio-Food Science, Gangneung-Wonju National University, Gangneung, Republic of Korea.

²Natural Product Informatics Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Republic of Korea.

³NVience Inc., Seoul, Republic of Korea.

⁴Natural Product Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Republic of Korea.

Corresponding Authors: Kwang Hyun Cha, Ki Young Choi

Abstract

Inflammatory bowel disease (IBD) is a chronic disorder characterized by recurrent gastrointestinal inflammation, lacking a precise aetiology and definitive cure. The gut microbiome is vital in preventing and treating IBD due to its various physiological functions. In the interplay between the gut microbiome and human health, extracellular vesicles secreted by gut bacteria (BEVs) are key mediators. Herein, we explore the role of Roseburia intestinalis (R)-derived EVs (R-EVs) as potent anti-inflammatory mediators in treating dextran sulfate sodium-induced colitis. R was selected as an optimal BEV producer for IBD treatment through ANCOM analysis. R-EVs with a 76 nm diameter were isolated from R using a tangential flow filtration system. Orally administered R-EVs effectively accumulated in inflamed colonic tissues and increased the abundance of Bifidobacterium on microbial changes, inhibiting colonic inflammation and prompting intestinal recovery. Due to the presence of Ile-Pro-Ile in the vesicular structure, R-EVs reduced the DPP4 activity in inflamed colonic tissue and increased the active GLP-1, thereby downregulating the NFκB and STAT3 via the PI3K pathway. Our results shed light on the impact of BEVs on intestinal recovery and gut microbiome alteration in treating IBD.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-22)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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