김다슬 (Havard medical school)

한빛사논문

조회266

김다슬Da-Seul Kim

Havard medical school

CV File 168 kb

ACS Nano, Aug 20 2024, | https://doi.org/10.1021/acsnano.4c05558 Hierarchical Chitin Nanocrystal-Based 3D Printed Dual-Layer Membranes Hydrogels: A Dual Drug Delivery Nano-Platform for Periodontal Tissue Regeneration

Danilo Martins dos Santos^a,b†, Jae-I Moon^c†, Da-Seul Kim^a†, Nicole Joy Bassous^a, Crisiane Aparecida Marangon^b, Sergio Paulo Campana-Filho^d, Daniel Souza Correa^b, Min-Ho Kang^e.f, Woo-Jin Kim^c*, Su Ryon Shin^a*

^aDivision of Engineering in Medicine, Department of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Cambridge, MA 02139, USA.

^bNanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentação, São Carlos, SP 13560-970, Brazil.

^cDepartment of Molecular Genetics, School of Dentistry and Dental Research Institute, Dental Multi-omics Center, Seoul National University, Seoul 03080, Republic of Korea.

^dSao Carlos Institute of Chemistry/University of São Paulo, Av. Trabalhador sao-carlense, 400, São Carlos, SP 13566-590, Brazil.

^eDepartment of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon 14662, Republic of Korea.

^fDepartment of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea.

^†These authors contributed equally to this work.

^*Corresponding Authors: Woo-Jin Kim, Su Ryon Shin

Abstract

Periodontitis, a prevalent chronic inflammatory disease caused by bacteria, poses a significant challenge to current treatments by merely slowing their progression. Herein, we propose an innovative solution in the form of hierarchical nanostructured 3D printed bilayer membranes that serve as dual-drug delivery nanoplatforms and provide scaffold function for the regeneration of periodontal tissue. Nanocomposite hydrogels were prepared by combining lipid nanoparticle-loaded grape seed extract and simvastatin, as well as chitin nanocrystals, which were then 3D printed into a bilayer membrane that possesses antimicrobial properties and multiscale porosity for periodontal tissue regeneration. The constructs exhibited excellent mechanical properties by adding chitin nanocrystals and provided a sustained release of distinct drugs over 24 days. We demonstrated that the bilayer membranes are cytocompatible and have the ability to induce bone-forming markers in human mesenchymal stem cells, while showing potent antibacterial activity against pathogens associated with periodontitis. In vivo studies further confirmed the efficacy of bilayer membranes in enhancing alveolar bone regeneration and reducing inflammation in a periodontal defect model. This approach suggests promising avenues for the development of implantable constructs that not only combat infections, but also promote the regeneration of periodontal tissue, providing valuable insights into advanced periodontitis treatment strategies.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-21)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오소재

댓글 작성 로그인

CV 열람하기

연락처 보기