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KAIST, IBS

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Nat. Commun., Aug 14 2024, 15 (1) | https://doi.org/10.1038/s41467-024-51461-z Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein

Changin Kim ^1,2,4, So Ri Yun ^1,2,4, Sang Jin Lee^1,2,4, Seong Ok Kim ^1,2,4, Hyosub Lee ^1,2, Jungkweon Choi ^1,2, Jong Goo Kim ^1,2, Tae Wu Kim ^1,2, Seyoung You ^1,2, Irina Kosheleva ³, Taeyoon Noh ^1,2, Jonghoon Baek ^1,2 & Hyotcherl Ihee ^1,2

¹Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.

²Center for Advanced Reaction Dynamics, Institute for Basic Science (IBS), Daejeon 34141, Republic of Korea.

³Center for Advanced Radiation Sources, The University of Chicago, Chicago, IL 60637, USA.

⁴These authors contributed equally: Changin Kim, So Ri Yun, Sang Jin Lee, Seong Ok Kim.

Corresponding author

Correspondence to Hyotcherl Ihee.

Abstract

The Light-oxygen-voltage-sensing domain (LOV) superfamily, found in enzymes and signal transduction proteins, plays a crucial role in converting light signals into structural signals, mediating various biological mechanisms. While time-resolved spectroscopic studies have revealed the dynamics of the LOV-domain chromophore's electronic structures, understanding the structural changes in the protein moiety, particularly regarding light-induced dimerization, remains challenging. Here, we utilize time-resolved X-ray liquidography to capture the light-induced dimerization of Avena sativa LOV2. Our analysis unveils that dimerization occurs within milliseconds after the unfolding of the A'α and Jα helices in the microsecond time range. Notably, our findings suggest that protein-protein interactions (PPIs) among the β-scaffolds, mediated by helix unfolding, play a key role in dimerization. In this work, we offer structural insights into the dimerization of LOV2 proteins following structural changes in the A'α and Jα helices, as well as mechanistic insights into the protein-protein association process driven by PPIs.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-19)
연구진국내 연구진
분야 생명과학 > 구조생물 및 생물물리학

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