유소영 (부산대학교)

한빛사논문

조회269

부산대학교

CV File 183 kb

Biomaterials, Aug 06 2024, 312 S0142-9612(24)00270-9 | https://doi.org/10.1016/j.biomaterials.2024.122736 Preventive and therapeutic effects of a super-multivalent sialylated filamentous bacteriophage against the influenza virus

Jinhyo Chung ^a,1, Sehoon Kim ^c,1, Jiyoon Jeong ^a, Doyeon Kim ^a, Anna Jo ^c, Hwa Young Kim^c, Jaehyeon Hwang ^a, Dae-Hyuk Kweon ^a, So Young Yoo ^c, Woo-Jae Chung ^a,b,d

^aDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea

^bCenter for Biologics, Sungkyunkwan University, Suwon, 16419, Republic of Korea

^cBIO-IT Foundry Technology Institute, Pusan National University, Busan, 46241, Republic of Korea

^dCenter for Study of Emerging and Re-emerging Viruses, Korea Virus Research Institute, Institute for Basic Science (IBS), Daejeon, 34126, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: So Young Yoo, Woo-Jae Chung

Abstract

The resurgence of influenza viruses as a significant global threat emphasizes the urgent need for innovative antiviral strategies beyond existing treatments. Here, we present the development and evaluation of a novel super-multivalent sialyllactosylated filamentous phage, termed t-6SLPhage, as a potent entry blocker for influenza A viruses. Structural variations in sialyllactosyl ligands, including linkage type, valency, net charge, and spacer length, were systematically explored to identify optimal binding characteristics against target hemagglutinins and influenza viruses. The selected SLPhage equipped with optimal ligands, exhibited exceptional inhibitory potency in in vitro infection inhibition assays. Furthermore, in vivo studies demonstrated its efficacy as both a preventive and therapeutic intervention, even when administered post-exposure at 2 days post-infection, under 4 lethal dose 50% conditions. Remarkably, co-administration with oseltamivir revealed a synergistic effect, suggesting potential combination therapies to enhance efficacy and mitigate resistance. Our findings highlight the efficacy and safety of sialylated filamentous bacteriophages as promising influenza inhibitors. Moreover, the versatility of M13 phages for surface modifications offers avenues for further engineering to enhance therapeutic and preventive performance.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-12)
연구진국내 연구진
분야 생명과학 > 생물공학

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