Thu-Hien Nguyen (전남대학교)

한빛사논문

조회111

전남대학교

New Phytol., Aug 07 2024, | https://doi.org/10.1111/nph.20029 The transcription factor LBD10 sustains pollen tube growth and integrity by modulating reactive oxygen species homeostasis via the regulation of flavonol biosynthesis in Arabidopsis

Thu-Hien Nguyen^1*, Min Jung Kim^2* and Jungmook Kim^1,2,3

¹Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, Korea;

²Department of Bioenergy Science and Technology, ChonnamNational University, Gwangju, 61186, Korea;

³Kumho Life Science Laboratory, Chonnam National University, Gwangju, 61186, Korea

^*These authors contributed equally to this work.

Author for correspondence: Jungmook Kim

Abstract

Reproduction in angiosperms relies on the precise growth of pollen tubes, facilitating the delivery of sperm cells to the ovule for double fertilization. LATERAL ORGAN BOUNDARIES DOMAIN10 (LBD10), a plant-specific transcription factor, plays a pivotal role in Arabidopsis pollen development. Here, we uncovered LBD10's function in sustaining pollen tube growth and integrity. The lbd10 mutant exhibited elevated levels of reactive oxygen species (ROS) and hydrogen peroxide (H₂O₂) in both pollen grains and tubes, leading to compromised pollen tube growth. The inhibition of ROS synthesis and scavenging of excess ROS with an antioxidant treatment each alleviated these defects in lbd10. The lbd10 mutant displayed reduced flavonol accumulation in both pollen grains and tubes. All the altered phenotypes of lbd10 were complemented by expressing LBD10 under its native promoter. Exogenous application of flavonoids recused the defects in pollen tube growth and integrity in lbd10, along with reducing the excess levels of ROS and H₂O₂. LBD10 directly binds the promoters of key flavonol biosynthesis genes in chromatin and promotes reporter gene expression in Arabidopsis mesophyll protoplasts. Our findings indicate that LBD10 modulates ROS homeostasis by transcriptionally activating genes crucial for flavonol biosynthesis, thereby maintaining pollen tube growth and integrity.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-09)
연구진국내 연구진
분야 생명과학 > 식물학

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