이승욱 (University of California, Berkeley)

한빛사논문

조회224

University of California, Berkeley

CV File 76 kb

Nano Lett., Aug 08 2024, | https://doi.org/10.1021/acs.nanolett.4c03231 Evolutionary Design of Self-Templated Supramolecular Fibrils Using M13 Bacteriophage for Tissue Engineering

Inseok Chae ^1,2, Woo-Jae Chung ^1,2, Hyo-Eon Jin^1,2, Robert J Yang¹, Han Kim ^2,3, Butaek Lim ^1,2, Hee Jung Lee ¹, Sun-Young Kim ⁴, Seung-Wuk Lee^1,2,3

¹Department of Bioengineering, University of California, Berkeley, California 94720, United States.

²Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

³Department of Applied Science and Technology, University of California, Berkeley, California 94720, United States.

⁴Department of Conservative Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea.

I.C. and W.-J.C. contributed equally to this manuscript.

Corresponding Author : Seung-Wuk Lee

Abstract

Biomaterials in nature form hierarchical structures and functions across various length scales through binding and assembly processes. Inspired by nature, we developed hierarchically organized tissue engineering materials through evolutionary screening and self-templating assembly. Leveraging the M13 bacteriophage (phage), we employed an evolutionary selection process against hydroxyapatite (HA) to isolate HA-binding phage (HAPh). The newly discovered phage exhibits a bimodal length, comprising 950 nm and 240 nm, where the synergistic effect of these dual lengths promotes the formation of supramolecular fibrils with periodic banded structures. The assembled HAPh fibrils show the capability of HA mineralization and the directional growth of osteoblast cells. When applied to a dentin surface, it induces the regeneration of dentin-like tissue structures, showcasing its potential applications as a scaffold in tissue engineering. The integration of evolutionary screening and self-templating assembly holds promise for the future development of hierarchically organized tissue engineering materials.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-09)
연구진국외 연구진
분야 바이오·의료융합 > 바이오소재

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