한빛사
>
한빛사논문
한빛사논문
- 조회75
J. Hazard. Mater., Jul 14 2024, 477 S0304-3894(24)01759-X | https://doi.org/10.1016/j.jhazmat.2024.135180
Air sampling and simultaneous detection of airborne influenza virus via gold nanorod-based plasmonic PCR
Kang Sik Nam a, Amin Piri a,b, Sangsoo Choi a, Jiwoo Jung a, Jungho Hwang a
aDepartment of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea
bInstitute of Engineering Research, Yonsei University, Seoul 03722, Republic of Korea
Correspondence to: Jungho Hwang
Abstract
Reliable and sensitive virus detection is essential to prevent airborne virus transmission. The polymerase chain reaction (PCR) is one of the most compelling and effective diagnostic techniques for detecting airborne pathogens. However, most PCR diagnostics rely on thermocycling, which involves a time-consuming Peltier block heating methodology. Plasmonic PCR is based on light-driven photothermal heating of plasmonic nanostructures to address the key drawbacks of traditional PCR. This study introduces a methodology for plasmonic PCR detection of air-sampled influenza virus (H1N1). An electrostatic air sampler was used to collect the aerosolized virus in a carrier liquid for 10 min. Simultaneously, the viruses collected in the liquid were transferred to a tube containing gold (Au) nanorods (aspect ratio = 3.6). H1N1 viruses were detected in 12 min, which is the total time required for reverse transcription, fast thermocycling via plasmonic heating through gold nanorods, and in situ fluorescence detection. This methodology showed a limit of detection of three RNA copies/μL liquid for H1N1 influenza virus, which is comparable to that of commercially available PCR devices. This methodology can be used for the rapid and precise identification of pathogens on-site, while significantly reducing the time required for monitoring airborne viruses.
논문정보
- Research article
- 2024년 07월 (BRIC 등록일 2024-08-06)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
